Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification

NWIPB OpenIR

	Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification
	Fu Yanyan 1; Song Cuihua 1; Qin Xueqin 1; Li Xiaoyan 1; Ding Mingzhou 1; Sun Zhiwei 2,3; Xia Lian 1,2,3; Suo Yourui 2; Li Yulin 2; You Jinmao 1,2
	2010-04-14
发表期刊	ACTA CHIMICA SINICA
ISSN	0567-7351
卷号	68 期号:7 页码:653-660
文章类型	Article
摘要	On a reversed-phased Hypersil C(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate (BAETS) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. Optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with BAETS at 95 degrees C for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. The identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. Correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/mL for the labeled bile acids.; On a reversed-phased Hypersil C(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate (BAETS) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. Optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with BAETS at 95 degrees C for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. The identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. Correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/mL for the labeled bile acids.
关键词	High Performance Liquid Chromatography/ion-trap Mass Spectrometry Percent Ionization 2-(12-oxobenzo[b]Acridin-5(12h)-yl)Ethyl 4-toluenesulfonate (Baets) Bile Acid
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; FATTY-ACIDS ; PRECOLUMN DERIVATIZATION ; CARBOXYLIC-ACIDS ; TRIFLUOROMETHANESULFONATE ; DERIVATIVES ; BRYOPHYTE ; REAGENT ; COLUMN ; SERUM
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000277418700009
引用统计
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1722
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fu Yanyan,Song Cuihua,Qin Xueqin,et al. Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification[J]. ACTA CHIMICA SINICA,2010,68(7):653-660.
APA	Fu Yanyan.,Song Cuihua.,Qin Xueqin.,Li Xiaoyan.,Ding Mingzhou.,...&You Jinmao.(2010).Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification.ACTA CHIMICA SINICA,68(7),653-660.
MLA	Fu Yanyan,et al."Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification".ACTA CHIMICA SINICA 68.7(2010):653-660.