Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers

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	Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers
	Xie, Xiaolong 1,2,3; Hu, Yanping 1,2; Wang, Li 1,2; Yang, Jian 1,2; Li, Yi 1; Peng, Min 1
	2009-09-15
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	8 期号:18 页码:4490-4494
文章类型	Article
摘要	Inter simple sequence repeat (ISSR) markers were used to detect the genetic diversity in three morphological types of cultivated Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao discovered in Gansu province of China. Eight primers used for analysis generated 165 scorable bands of which 162 (98.18%) were polymorphic. The percentage polymorphic bands (PPB), Shannon's information index (I) and Nei's gene diversity (h) for 3 types ranged from 59.39%, 0.2912, 0.1901 to 78.18%, 0.3411, 0.2238 respectively, indicating each type had a high genetic diversity. Analysis of molecular variance (AMOVA) showed 90.41% of the total genetic diversity existed within the 3 morphological types, whereas only 9.59% occurred among them. The UPGMA tree based on SM similarity coefficients implied that the 3 types had genetically differentiated though not obviously. The high genetic diversity suggested that each type had a potential to develop new strains or cultivars and ISSR was a potentially useful tool in the process of selective breeding of A. membranaceus var. mongholicus.; Inter simple sequence repeat (ISSR) markers were used to detect the genetic diversity in three morphological types of cultivated Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao discovered in Gansu province of China. Eight primers used for analysis generated 165 scorable bands of which 162 (98.18%) were polymorphic. The percentage polymorphic bands (PPB), Shannon's information index (I) and Nei's gene diversity (h) for 3 types ranged from 59.39%, 0.2912, 0.1901 to 78.18%, 0.3411, 0.2238 respectively, indicating each type had a high genetic diversity. Analysis of molecular variance (AMOVA) showed 90.41% of the total genetic diversity existed within the 3 morphological types, whereas only 9.59% occurred among them. The UPGMA tree based on SM similarity coefficients implied that the 3 types had genetically differentiated though not obviously. The high genetic diversity suggested that each type had a potential to develop new strains or cultivars and ISSR was a potentially useful tool in the process of selective breeding of A. membranaceus var. mongholicus.
关键词	Astragalus Membranaceus Var. Mongholicus Morphological Type Genetic Diversity Issr Radix Astragali
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	ISSR MARKERS ; POPULATIONS ; CHINA ; RAPD
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000270306500028
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1826
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Henan Univ Tradit Chinese Med, Zhengzhou 450008, Peoples R China
推荐引用方式 GB/T 7714	Xie, Xiaolong,Hu, Yanping,Wang, Li,et al. Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(18):4490-4494.
APA	Xie, Xiaolong,Hu, Yanping,Wang, Li,Yang, Jian,Li, Yi,&Peng, Min.(2009).Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(18),4490-4494.
MLA	Xie, Xiaolong,et al."Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.18(2009):4490-4494.