NWIPB OpenIR
Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC
Liu, Hongliu1; Gong, Chengcheng1; Liao, Zhixin1; Chen, Tao2; Li, Yulin2
2017-11-01
发表期刊CHROMATOGRAPHIA
卷号80期号:11页码:1651-1658
文章类型Article
摘要An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by H-1-NMR and C-13-NMR.
关键词Molecularly Imprinted Solid-phase Extraction Hsccc Clematis Akebioides Caffeic Acid Separation
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1007/s10337-017-3406-z
关键词[WOS]COUNTER-CURRENT CHROMATOGRAPHY ; DISTILLATION-PRECIPITATION POLYMERIZATION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PREPARATIVE SEPARATION ; SENSITIVE DETECTION ; IONIC LIQUIDS ; ACID ; POLYMERS ; SYSTEM
收录类别SCI
语种英语
项目资助者priority academic program development of Jiangsu higher Education Institutions(1107047002) ; Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University)(CMEMR2016-B06) ; Instrument Function Development Project of CAS(2017g107)
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000415157600006
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/10361
专题中国科学院西北高原生物研究所
作者单位1.Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
推荐引用方式
GB/T 7714
Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC[J]. CHROMATOGRAPHIA,2017,80(11):1651-1658.
APA Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,Chen, Tao,&Li, Yulin.(2017).Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.CHROMATOGRAPHIA,80(11),1651-1658.
MLA Liu, Hongliu,et al."Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC".CHROMATOGRAPHIA 80.11(2017):1651-1658.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Hongliu]的文章
[Gong, Chengcheng]的文章
[Liao, Zhixin]的文章
百度学术
百度学术中相似的文章
[Liu, Hongliu]的文章
[Gong, Chengcheng]的文章
[Liao, Zhixin]的文章
必应学术
必应学术中相似的文章
[Liu, Hongliu]的文章
[Gong, Chengcheng]的文章
[Liao, Zhixin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。