Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC | |
Liu, Hongliu1; Gong, Chengcheng1; Liao, Zhixin1; Chen, Tao2; Li, Yulin2 | |
2017-11-01 | |
发表期刊 | CHROMATOGRAPHIA |
ISSN | 0009-5893 |
卷号 | 80期号:11页码:1651-1658 |
文章类型 | Article |
摘要 | An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by H-1-NMR and C-13-NMR. |
关键词 | Molecularly Imprinted Solid-phase Extraction Hsccc Clematis Akebioides Caffeic Acid Separation |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1007/s10337-017-3406-z |
关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; DISTILLATION-PRECIPITATION POLYMERIZATION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PREPARATIVE SEPARATION ; SENSITIVE DETECTION ; IONIC LIQUIDS ; ACID ; POLYMERS ; SYSTEM |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | priority academic program development of Jiangsu higher Education Institutions(1107047002) ; Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University)(CMEMR2016-B06) ; Instrument Function Development Project of CAS(2017g107) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000415157600006 |
出版者 | SPRINGER HEIDELBERG |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/10361 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Liao, Zhixin; Li, Yulin |
作者单位 | 1.Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC[J]. CHROMATOGRAPHIA,2017,80(11):1651-1658. |
APA | Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,Chen, Tao,&Li, Yulin.(2017).Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.CHROMATOGRAPHIA,80(11),1651-1658. |
MLA | Liu, Hongliu,et al."Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC".CHROMATOGRAPHIA 80.11(2017):1651-1658. |
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