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Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC
Liu, Hongliu1; Gong, Chengcheng1; Liao, Zhixin1; Chen, Tao2; Li, Yulin2
2017-11-01
Source PublicationCHROMATOGRAPHIA
ISSN0009-5893
Volume80Issue:11Pages:1651-1658
SubtypeArticle
AbstractAn efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by H-1-NMR and C-13-NMR.
KeywordMolecularly Imprinted Solid-phase Extraction Hsccc Clematis Akebioides Caffeic Acid Separation
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1007/s10337-017-3406-z
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; DISTILLATION-PRECIPITATION POLYMERIZATION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PREPARATIVE SEPARATION ; SENSITIVE DETECTION ; IONIC LIQUIDS ; ACID ; POLYMERS ; SYSTEM
Indexed BySCI
Language英语
Funding Organizationpriority academic program development of Jiangsu higher Education Institutions(1107047002) ; Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University)(CMEMR2016-B06) ; Instrument Function Development Project of CAS(2017g107)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000415157600006
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/10361
Collection中国科学院西北高原生物研究所
Corresponding AuthorLiao, Zhixin; Li, Yulin
Affiliation1.Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC[J]. CHROMATOGRAPHIA,2017,80(11):1651-1658.
APA Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,Chen, Tao,&Li, Yulin.(2017).Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.CHROMATOGRAPHIA,80(11),1651-1658.
MLA Liu, Hongliu,et al."Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC".CHROMATOGRAPHIA 80.11(2017):1651-1658.
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