LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent

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	LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
	Ding, Chenxu 1,4; Wang, Lingyun 1,5; Tian, Chunliang 2; Li, Yulin 1; Sun, Zhiwei 1,5; Wang, Honglun 1; Suo, Yourui 1; You, Jinmao 1,3
	2008-12-01
发表期刊	CHROMATOGRAPHIA
卷号	68 期号:11-12 页码:893-902
文章类型	Article
摘要	A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.
关键词	Column Liquid Chromatography-mass Spectrometry Carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域	生物科学
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; FAST-ATOM-BOMBARDMENT ; CAPILLARY-ELECTROPHORESIS ; OLIGOSACCHARIDES ; DERIVATIVES ; SUGARS ; ACID ; GLYCOPROTEINS ; ELECTROSPRAY
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000261422300003
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1187
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Jining Univ, Dept Chem, Jining 273155, Shandong, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 4.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ding, Chenxu,Wang, Lingyun,Tian, Chunliang,et al. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent[J]. CHROMATOGRAPHIA,2008,68(11-12):893-902.
APA	Ding, Chenxu.,Wang, Lingyun.,Tian, Chunliang.,Li, Yulin.,Sun, Zhiwei.,...&You, Jinmao.(2008).LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA,68(11-12),893-902.
MLA	Ding, Chenxu,et al."LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent".CHROMATOGRAPHIA 68.11-12(2008):893-902.

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