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LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
Ding, Chenxu1,4; Wang, Lingyun1,5; Tian, Chunliang2; Li, Yulin1; Sun, Zhiwei1,5; Wang, Honglun1; Suo, Yourui1; You, Jinmao1,3
2008-12-01
Source PublicationCHROMATOGRAPHIA
Volume68Issue:11-12Pages:893-902
SubtypeArticle
AbstractA novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.
KeywordColumn Liquid Chromatography-mass Spectrometry Carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Subject Area生物科学
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; FAST-ATOM-BOMBARDMENT ; CAPILLARY-ELECTROPHORESIS ; OLIGOSACCHARIDES ; DERIVATIVES ; SUGARS ; ACID ; GLYCOPROTEINS ; ELECTROSPRAY
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000261422300003
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1187
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Jining Univ, Dept Chem, Jining 273155, Shandong, Peoples R China
3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
4.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China
5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Ding, Chenxu,Wang, Lingyun,Tian, Chunliang,et al. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent[J]. CHROMATOGRAPHIA,2008,68(11-12):893-902.
APA Ding, Chenxu.,Wang, Lingyun.,Tian, Chunliang.,Li, Yulin.,Sun, Zhiwei.,...&You, Jinmao.(2008).LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA,68(11-12),893-902.
MLA Ding, Chenxu,et al."LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent".CHROMATOGRAPHIA 68.11-12(2008):893-902.
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