NWIPB OpenIR
LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
Ding, Chenxu1,4; Wang, Lingyun1,5; Tian, Chunliang2; Li, Yulin1; Sun, Zhiwei1,5; Wang, Honglun1; Suo, Yourui1; You, Jinmao1,3
2008-12-01
发表期刊CHROMATOGRAPHIA
卷号68期号:11-12页码:893-902
文章类型Article
摘要A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape bee pollen samples is reported. Carbohydrates are derivatized to their bis-NMP-labeled derivatives. Derivatives showed an intense protonated molecular ion at m/z [M+H](+) in positive-ion detection mode. The mass-to-charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative analysis of carbohydrates. This characteristic fragment ion is from the cleavage of C2-C3 bond in carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose and glyceraldehydes and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids such as galacturonic acid and glucuronic acid (m = n - 2, n is carbon number of carbohydrate). No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed due to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed-phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected.
关键词Column Liquid Chromatography-mass Spectrometry Carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域生物科学
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; FAST-ATOM-BOMBARDMENT ; CAPILLARY-ELECTROPHORESIS ; OLIGOSACCHARIDES ; DERIVATIVES ; SUGARS ; ACID ; GLYCOPROTEINS ; ELECTROSPRAY
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000261422300003
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1187
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Jining Univ, Dept Chem, Jining 273155, Shandong, Peoples R China
3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
4.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China
5.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Ding, Chenxu,Wang, Lingyun,Tian, Chunliang,et al. LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent[J]. CHROMATOGRAPHIA,2008,68(11-12):893-902.
APA Ding, Chenxu.,Wang, Lingyun.,Tian, Chunliang.,Li, Yulin.,Sun, Zhiwei.,...&You, Jinmao.(2008).LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent.CHROMATOGRAPHIA,68(11-12),893-902.
MLA Ding, Chenxu,et al."LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent".CHROMATOGRAPHIA 68.11-12(2008):893-902.
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