Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection | |
| Zhao Wen-Chen2,3; Liu Ling-Jun2; Zhao Xian-En1; Ding Chen-Xu1; Suo You-Rui1; You Jin-Mao1,2 | |
| 2008-08-01 | |
| 发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 卷号 | 36期号:8页码:1071-1076 |
| 文章类型 | Article |
| 摘要 | On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.; On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids. |
| 关键词 | High Performance Liquid Chromatography/mass Spectrometry Fluorescence Detection Pre-column Derivatization Amino Acids Bee Pollen |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科领域 | 生物科学 |
| 关键词[WOS] | IONIZATION MASS-SPECTROMETRY ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; CHLOROFORMATE ; IDENTIFICATION ; HYDROLYSATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000260136800012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1189 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 3.Lu Nan Pharm Grp Joint Stock Ltd, Linyi 276005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,et al. Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1071-1076. |
| APA | Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,Ding Chen-Xu,Suo You-Rui,&You Jin-Mao.(2008).Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(8),1071-1076. |
| MLA | Zhao Wen-Chen,et al."Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.8(2008):1071-1076. |
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