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Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells
Shi, Ping1,2; Huang, Zhiwei1,3; Chen, Guichen1
2008
发表期刊AMERICAN JOURNAL OF CHINESE MEDICINE
卷号36期号:4页码:805-813
文章类型Article
摘要Rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. In this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma BEL-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. Using MTT assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of BEL-7402 cell proliferation in a concentration- and time-dependent manner. The increase of apoptosis rate was observed after incubation of BEL-7402 cells with rhein at 50-200 mu M for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. Moreover, rhein-induced cell cycle S-phase arrest. Additionally, after rhein treatment, expression levels of c-Myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time PCR assay. The results demonstrate for the first time that cell cycle S-phase arrest is one of the mechanisms of rhein in inhibition of BEL-7402 cells. Rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-Myc and apoptosis through the caspase-dependent pathway. It is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future.; Rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. In this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma BEL-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. Using MTT assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of BEL-7402 cell proliferation in a concentration- and time-dependent manner. The increase of apoptosis rate was observed after incubation of BEL-7402 cells with rhein at 50-200 mu M for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. Moreover, rhein-induced cell cycle S-phase arrest. Additionally, after rhein treatment, expression levels of c-Myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time PCR assay. The results demonstrate for the first time that cell cycle S-phase arrest is one of the mechanisms of rhein in inhibition of BEL-7402 cells. Rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-Myc and apoptosis through the caspase-dependent pathway. It is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future.
关键词Rhein Human Hepatocelluar Carcinoma Bel-7402 Cells Apoptosis Cell Cycle Arrest C-myc Caspase-3
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科领域药学
关键词[WOS]NATURAL-PRODUCTS ; CANCER
收录类别SCI
语种英语
WOS研究方向Integrative & Complementary Medicine ; General & Internal Medicine
WOS类目Integrative & Complementary Medicine ; Medicine, General & Internal
WOS记录号WOS:000258793200016
引用统计
被引频次:68[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1197
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China
2.Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 USA
3.Baylor Coll Med, Houston, TX 77030 USA
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Shi, Ping,Huang, Zhiwei,Chen, Guichen. Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2008,36(4):805-813.
APA Shi, Ping,Huang, Zhiwei,&Chen, Guichen.(2008).Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.AMERICAN JOURNAL OF CHINESE MEDICINE,36(4),805-813.
MLA Shi, Ping,et al."Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells".AMERICAN JOURNAL OF CHINESE MEDICINE 36.4(2008):805-813.
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