Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification | |
Zhao, Xianen1,2; Suo, Yourui1,2; Wang, Lingyun1,2; You, Jinmao1,3; Ding, Chenxu1,3 | |
2008 | |
发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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卷号 | 31期号:16页码:2375-2400 |
文章类型 | Article |
摘要 | A new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP), coupling with liquid chromatography (LC) with electrospray ionization mass spectrometry (ESI-MS) for the detection of carbohydrates from a famous Tibetan medicine is reported. Carbohydrates were derivatized to their bis-NMP-labeled derivatives. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by UV and ESI-MS. Derivatives showed intense protonated molecular ion at m/z [M+H]+ in positive ion mode. The mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of C2-C3 bond in the carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose, and glyceraldehydes, and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). Compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent, currently synthesized NMP show the advantage of higher sensitivity to carbohydrate compounds with UV and ESI-MS detection.; A new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP), coupling with liquid chromatography (LC) with electrospray ionization mass spectrometry (ESI-MS) for the detection of carbohydrates from a famous Tibetan medicine is reported. Carbohydrates were derivatized to their bis-NMP-labeled derivatives. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by UV and ESI-MS. Derivatives showed intense protonated molecular ion at m/z [M+H]+ in positive ion mode. The mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of C2-C3 bond in the carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose, and glyceraldehydes, and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). Compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent, currently synthesized NMP show the advantage of higher sensitivity to carbohydrate compounds with UV and ESI-MS detection. |
关键词 | 1-(2-naphthyl)-3-methyl-5-pyrazolone (Nmp) Carbohydrates Derivatization Herpetospermum Pedunculosum (Ser.) Baill Liquid Chromatography-mass Spectrometry (Esi/ms) |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
学科领域 | 生物科学 |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HERPETOSPERMUM-CAUDIGERUM WALL ; DESORPTION/IONIZATION MASS-SPECTROMETRY ; OLIGOSACCHARIDES ; DERIVATIVES ; DERIVATIZATION ; ELECTROSPRAY ; SEPARATION ; ACID ; MONOSACCHARIDE |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000258622400002 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1198 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Xianen,Suo, Yourui,Wang, Lingyun,et al. Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2008,31(16):2375-2400. |
APA | Zhao, Xianen,Suo, Yourui,Wang, Lingyun,You, Jinmao,&Ding, Chenxu.(2008).Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,31(16),2375-2400. |
MLA | Zhao, Xianen,et al."Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 31.16(2008):2375-2400. |
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