Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification

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	Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification
	Zhao, Xianen 1,2; Suo, Yourui 1,2; Wang, Lingyun 1,2; You, Jinmao 1,3; Ding, Chenxu 1,3
	2008
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号	31 期号:16 页码:2375-2400
文章类型	Article
摘要	A new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP), coupling with liquid chromatography (LC) with electrospray ionization mass spectrometry (ESI-MS) for the detection of carbohydrates from a famous Tibetan medicine is reported. Carbohydrates were derivatized to their bis-NMP-labeled derivatives. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by UV and ESI-MS. Derivatives showed intense protonated molecular ion at m/z [M+H]+ in positive ion mode. The mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of C2-C3 bond in the carbohydrate chain giving the specific fragment ions at m/z [MH-CmH2m+1Om-H2O](+) for pentose, hexose, and glyceraldehydes, and at m/z [MH-CmH2m-1Om+1-H2O](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). Compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent, currently synthesized NMP show the advantage of higher sensitivity to carbohydrate compounds with UV and ESI-MS detection.; A new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP), coupling with liquid chromatography (LC) with electrospray ionization mass spectrometry (ESI-MS) for the detection of carbohydrates from a famous Tibetan medicine is reported. Carbohydrates were derivatized to their bis-NMP-labeled derivatives. The method, in conjunction with a gradient elution, offered a baseline resolution of carbohydrate derivatives on a reversed phase Hypersil ODS-2 column. The carbohydrates such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose, and fucose could be successfully detected by UV and ESI-MS. Derivatives showed intense protonated molecular ion at m/z [M+H]+ in positive ion mode. The mass to charge ratios of characteristic fragment ions at m/z 473.0 could be used for the accurately qualitative identification of carbohydrates; this characteristic fragment ion was from the cleavage of C2-C3 bond in the carbohydrate chain giving the specific fragment ions at m/z [MH-C(m)H(2m+1)O(m)-H(2)O](+) for pentose, hexose, and glyceraldehydes, and at m/z [MH-C(m)H(2m-1)O(m+1)-H(2)O](+) for alduronic acids, such as galacturonic acid and glucuronic acid (m=n-2, n is carbon atom number of carbohydrate). Compared with the traditional 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent, currently synthesized NMP show the advantage of higher sensitivity to carbohydrate compounds with UV and ESI-MS detection.
关键词	1-(2-naphthyl)-3-methyl-5-pyrazolone (Nmp) Carbohydrates Derivatization Herpetospermum Pedunculosum (Ser.) Baill Liquid Chromatography-mass Spectrometry (Esi/ms)
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域	生物科学
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; HERPETOSPERMUM-CAUDIGERUM WALL ; DESORPTION/IONIZATION MASS-SPECTROMETRY ; OLIGOSACCHARIDES ; DERIVATIVES ; DERIVATIZATION ; ELECTROSPRAY ; SEPARATION ; ACID ; MONOSACCHARIDE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000258622400002
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1198
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xianen,Suo, Yourui,Wang, Lingyun,et al. Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2008,31(16):2375-2400.
APA	Zhao, Xianen,Suo, Yourui,Wang, Lingyun,You, Jinmao,&Ding, Chenxu.(2008).Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,31(16),2375-2400.
MLA	Zhao, Xianen,et al."Analysis of carbohydrates in a tibetan medicine using new labeling reagent, 1-(2-naphthyl)-3-methyl-5-pyrazolone, by HPLC with DAD detection and ESI-MS identification".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 31.16(2008):2375-2400.