Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae) | |
| Wang, Ailan1; Zhang, Qian1; Wan, Dongshi1; Yang, Yanzhuo1; Liu, Jianquan1,2 | |
| 2008 | |
| 发表期刊 | CONSERVATION GENETICS
 |
| 卷号 | 9期号:4页码:969-971 |
| 文章类型 | Article |
| 摘要 | Hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest China. The wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.; Hippophae rhamnoides ssp. sinensis occurs mainly in the and regions of northwest China. The wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies. |
| 关键词 | Hippophae Rhamnoides Ssp Sinensis Microsatellite Markers Population Genetics |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科领域 | 生物科学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biodiversity & Conservation ; Genetics & Heredity |
| WOS类目 | Biodiversity Conservation ; Genetics & Heredity |
| WOS记录号 | WOS:000257420700022 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1207 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Qinghai Tibetan Plateau Biol Evolut & Ada, Xining, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ailan,Zhang, Qian,Wan, Dongshi,et al. Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)[J]. CONSERVATION GENETICS,2008,9(4):969-971. |
| APA | Wang, Ailan,Zhang, Qian,Wan, Dongshi,Yang, Yanzhuo,&Liu, Jianquan.(2008).Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae).CONSERVATION GENETICS,9(4),969-971. |
| MLA | Wang, Ailan,et al."Nine microsatellite DNA primers for Hippophae rhamnoides ssp sinensis (Elaeagnaceae)".CONSERVATION GENETICS 9.4(2008):969-971. |
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