NWIPB OpenIR
Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia
Li, Z. X.1,3; Zhang, X. Q.1; Zhang, H. G.1,2; Cao, S. H.1; Wang, D. W.1; Hao, S. T.1; Li, L. H.4; Li, H. J.4; Wang, X. P.1
2008-05-01
Source PublicationJOURNAL OF CEREAL SCIENCE
Volume47Issue:3Pages:429-437
SubtypeArticle
AbstractThe x- and y-type high molecular weight (HMW) glutenin subunits are conserved seed storage proteins in wheat and related species. Here we describe investigations on the HMW glutenin subunits from several Pseudoroegneria accessions. The electrophoretic mobilities of the HMW glutenin subunits from Pd. stipifolia, Pd tauri and Pd strigosa were much faster than those of orthologous wheat subunits, indicating that their protein size may be smaller than that of wheat subunits. The coding sequence of the Glu-1St1 subunit (encoded by the Pseudoroegneria stipifolia accession PI325181) was isolated, and found to represent the native open reading frame (ORF) by in vitro expression. The deduced amino acid sequence of Glu-1St1 matched with that determined from the native subunit by mass spectrometric analysis. The domain organization in Glu-1St1 showed high similarity with that of typical HMW glutenin subunits. However, Glu-1St1 exhibited several distinct characteristics. First, the length of its repetitive domain was substantially smaller than that of conventional subunits, which explains its much faster electrophoretic mobility in SDS-PAGE. Second, although the N-terminal domain of Glu-1St1 resembled that of y-type subunit, its C-terminal domain was more similar to that of x-type subunit. Third, the N- and C-terminat domains of Glu-1St1 shared conserved features with those of barley D-hordein, but the repeat motifs and the organization of its repetitive domain were more similar to those of HMW glutenin subunits than to D-hordein. We conclude that Glu-1St1 is a novel variant of HMW glutenin subunits. The analysis of Glu-1St1 may provide new insight into the evolution of HMW glutenin subunits in Triticeae species. (C) 2007 Elsevier Ltd. All rights reserved.; The x- and y-type high molecular weight (HMW) glutenin subunits are conserved seed storage proteins in wheat and related species. Here we describe investigations on the HMW glutenin subunits from several Pseudoroegneria accessions. The electrophoretic mobilities of the HMW glutenin subunits from Pd. stipifolia, Pd tauri and Pd strigosa were much faster than those of orthologous wheat subunits, indicating that their protein size may be smaller than that of wheat subunits. The coding sequence of the Glu-1St1 subunit (encoded by the Pseudoroegneria stipifolia accession PI325181) was isolated, and found to represent the native open reading frame (ORF) by in vitro expression. The deduced amino acid sequence of Glu-1St1 matched with that determined from the native subunit by mass spectrometric analysis. The domain organization in Glu-1St1 showed high similarity with that of typical HMW glutenin subunits. However, Glu-1St1 exhibited several distinct characteristics. First, the length of its repetitive domain was substantially smaller than that of conventional subunits, which explains its much faster electrophoretic mobility in SDS-PAGE. Second, although the N-terminal domain of Glu-1St1 resembled that of y-type subunit, its C-terminal domain was more similar to that of x-type subunit. Third, the N- and C-terminat domains of Glu-1St1 shared conserved features with those of barley D-hordein, but the repeat motifs and the organization of its repetitive domain were more similar to those of HMW glutenin subunits than to D-hordein. We conclude that Glu-1St1 is a novel variant of HMW glutenin subunits. The analysis of Glu-1St1 may provide new insight into the evolution of HMW glutenin subunits in Triticeae species. (C) 2007 Elsevier Ltd. All rights reserved.
KeywordPseudoroegneria Stipifolia Hmw Glutenin Subunit D-hordein Wheat Phylogenetic Analysis Evolution
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Subject Area生物科学
WOS KeywordDESORPTION/IONISATION MASS-SPECTROMETRY ; MOLECULAR-WEIGHT GLUTENIN ; STORAGE PROTEINS ; WHEAT ; REGIONS ; IDENTIFICATION ; SEQUENCE ; ALLELES ; BARLEY ; LOCUS
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000256601200005
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1210
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
2.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Li, Z. X.,Zhang, X. Q.,Zhang, H. G.,et al. Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia[J]. JOURNAL OF CEREAL SCIENCE,2008,47(3):429-437.
APA Li, Z. X..,Zhang, X. Q..,Zhang, H. G..,Cao, S. H..,Wang, D. W..,...&Wang, X. P..(2008).Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia.JOURNAL OF CEREAL SCIENCE,47(3),429-437.
MLA Li, Z. X.,et al."Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia".JOURNAL OF CEREAL SCIENCE 47.3(2008):429-437.
Files in This Item: Download All
File Name/Size DocType Version Access License
Isolation and charac(426KB) 开放获取LicenseView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Z. X.]'s Articles
[Zhang, X. Q.]'s Articles
[Zhang, H. G.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Z. X.]'s Articles
[Zhang, X. Q.]'s Articles
[Zhang, H. G.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Z. X.]'s Articles
[Zhang, X. Q.]'s Articles
[Zhang, H. G.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.