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	Separation and determination of flavone and xanthone glycosides in Tibetan folk medicinal species Swertia mussotii and S-franchetiana by capillary electrophoresis
	Li, Y. -L.1,3; Ding, C. -X.1,3; Wang, H. -L.1; Suo, Y. -R.1; You, J. -M.2; Chen, G. -C.1
	2008-06-01
发表期刊	JOURNAL OF ANALYTICAL CHEMISTRY
卷号	63期号:6页码:574-579
文章类型	Article
摘要	The contents of five pharmacologically active flavone and xanthone glycosides, namely, swertianolin, swertisin, isoorientin, mangiferin, and 7-O-[alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone, extracted from Tibetan folk medicinal species Swertia mussotii and S. franchetiana were determined by capillary electrophoresis with diode-array detection. The separation of five components has been optimized with a capillary column with a total length of 48.5 cm and effective length of 40 cm (50 mu m i.d). The influence of the running buffer, the sodium dodecyl sulfonate (SDS) concentration, organic modifier, etc. on the resolution was evaluated. The background electrolyte contained 30 mM borate buffer, 28 mM SDS, 1.0% (v/v) acetonitrile, and was adjusted to pH 9.0 with 0.1 M NaOH. A good baseline resolution was obtained for the separation of five components within 5 min with the working voltage of 24 kV and a column temperature of 25 degrees C. The established method was rapid and reproducible for the separation and determination of five flavone and xanthone glycosides from the extracts of S. mussotii and franchetiana plant samples.; The contents of five pharmacologically active flavone and xanthone glycosides, namely, swertianolin, swertisin, isoorientin, mangiferin, and 7-O-[alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone, extracted from Tibetan folk medicinal species Swertia mussotii and S. franchetiana were determined by capillary electrophoresis with diode-array detection. The separation of five components has been optimized with a capillary column with a total length of 48.5 cm and effective length of 40 cm (50 mu m i.d). The influence of the running buffer, the sodium dodecyl sulfonate (SDS) concentration, organic modifier, etc. on the resolution was evaluated. The background electrolyte contained 30 mM borate buffer, 28 mM SDS, 1.0% (v/v) acetonitrile, and was adjusted to pH 9.0 with 0.1 M NaOH. A good baseline resolution was obtained for the separation of five components within 5 min with the working voltage of 24 kV and a column temperature of 25 degrees C. The established method was rapid and reproducible for the separation and determination of five flavone and xanthone glycosides from the extracts of S. mussotii and franchetiana plant samples.
关键词	Pharmacologically Active Xanthones Securidaca-inappendiculata Optimized Separation Zone-electrophoresis Gentianaceae Constituents
WOS标题词	Science & Technology ; Physical Sciences
学科领域	生物科学
关键词[WOS]	PHARMACOLOGICALLY ACTIVE XANTHONES ; SECURIDACA-INAPPENDICULATA ; OPTIMIZED SEPARATION ; ZONE-ELECTROPHORESIS ; GENTIANACEAE ; CONSTITUENTS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000256340000010
引用统计	被引频次：8[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1211
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Dept Chem, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Y. -L.,Ding, C. -X.,Wang, H. -L.,et al. Separation and determination of flavone and xanthone glycosides in Tibetan folk medicinal species Swertia mussotii and S-franchetiana by capillary electrophoresis[J]. JOURNAL OF ANALYTICAL CHEMISTRY,2008,63(6):574-579.
APA	Li, Y. -L.,Ding, C. -X.,Wang, H. -L.,Suo, Y. -R.,You, J. -M.,&Chen, G. -C..(2008).Separation and determination of flavone and xanthone glycosides in Tibetan folk medicinal species Swertia mussotii and S-franchetiana by capillary electrophoresis.JOURNAL OF ANALYTICAL CHEMISTRY,63(6),574-579.
MLA	Li, Y. -L.,et al."Separation and determination of flavone and xanthone glycosides in Tibetan folk medicinal species Swertia mussotii and S-franchetiana by capillary electrophoresis".JOURNAL OF ANALYTICAL CHEMISTRY 63.6(2008):574-579.

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