Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae) | |
| Zhang, Dong-Yuan1,2; Chen, Na1,2; Yang, Yan-Zhuo1,2; Zhang, Qian1,2; Liu, Jian-Quan1,2 | |
| 2008 | |
| 发表期刊 | CONSERVATION GENETICS
 |
| 卷号 | 9期号:2页码:475-477 |
| 文章类型 | Article |
| 摘要 | Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with R. tanguticum. These microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species.; Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with R. tanguticum. These microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species. |
| 关键词 | Rhubarb Rheum Tanguticum Microsatellite Markers Genetic Diversity Conservation |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科领域 | 遗传学 |
| 关键词[WOS] | MORPHOLOGY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biodiversity & Conservation ; Genetics & Heredity |
| WOS类目 | Biodiversity Conservation ; Genetics & Heredity |
| WOS记录号 | WOS:000254242400026 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1227 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, MOE Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,et al. Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)[J]. CONSERVATION GENETICS,2008,9(2):475-477. |
| APA | Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,Zhang, Qian,&Liu, Jian-Quan.(2008).Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae).CONSERVATION GENETICS,9(2),475-477. |
| MLA | Zhang, Dong-Yuan,et al."Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)".CONSERVATION GENETICS 9.2(2008):475-477. |
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