Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV) | |
| You, Jinmao1,2; Sheng, Xiao2; Ding, Chenxu1; Sun, Zhiwei2; Suo, Yourui1; Wang, Honglun1; Li, Win1 | |
| 2008-02-18 | |
| 发表期刊 | ANALYTICA CHIMICA ACTA
 |
| 卷号 | 609期号:1页码:66-75 |
| 文章类型 | Article |
| 摘要 | A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupled with capillary electrophoresis (CE) with DAD detection for the determination of carbohydrates has been developed. The chromophore in the 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) L mol(-1) cm(-1)). This pen-nits NMP-labeled carbohydrates to be detected with UV absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. The method for the derivatization. of carbohydrates with NMP is simplified. The derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. Nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in CE mode. Good reproducibility can be obtained with relative standard deviation (R.S.D.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. Furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (C) 2008 Published by Elsevier B.V.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupled with capillary electrophoresis (CE) with DAD detection for the determination of carbohydrates has been developed. The chromophore in the 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) L mol(-1) cm(-1)). This pen-nits NMP-labeled carbohydrates to be detected with UV absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. The method for the derivatization. of carbohydrates with NMP is simplified. The derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. Nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in CE mode. Good reproducibility can be obtained with relative standard deviation (R.S.D.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. Furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (C) 2008 Published by Elsevier B.V. |
| 关键词 | Capillary Electrophoresis (Ce) Carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone (Nmp) Derivatization |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科领域 | 生物科学 |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; DESORPTION/IONIZATION MASS-SPECTROMETRY ; FAST-ATOM-BOMBARDMENT ; 1-PHENYL-3-METHYL-5-PYRAZOLONE DERIVATIVES ; STRUCTURAL CHARACTERIZATION ; SUGAR CHAINS ; OLIGOSACCHARIDES ; ACID ; SEPARATION ; GLYCOPROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000253560800008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1234 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Sheng, Xiao,Ding, Chenxu,et al. Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)[J]. ANALYTICA CHIMICA ACTA,2008,609(1):66-75. |
| APA | You, Jinmao.,Sheng, Xiao.,Ding, Chenxu.,Sun, Zhiwei.,Suo, Yourui.,...&Li, Win.(2008).Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV).ANALYTICA CHIMICA ACTA,609(1),66-75. |
| MLA | You, Jinmao,et al."Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)".ANALYTICA CHIMICA ACTA 609.1(2008):66-75. |
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