NWIPB OpenIR
Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)
You, Jinmao1,2; Sheng, Xiao2; Ding, Chenxu1; Sun, Zhiwei2; Suo, Yourui1; Wang, Honglun1; Li, Win1
2008-02-18
发表期刊ANALYTICA CHIMICA ACTA
卷号609期号:1页码:66-75
文章类型Article
摘要A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupled with capillary electrophoresis (CE) with DAD detection for the determination of carbohydrates has been developed. The chromophore in the 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) L mol(-1) cm(-1)). This pen-nits NMP-labeled carbohydrates to be detected with UV absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. The method for the derivatization. of carbohydrates with NMP is simplified. The derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. Nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in CE mode. Good reproducibility can be obtained with relative standard deviation (R.S.D.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. Furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (C) 2008 Published by Elsevier B.V.; A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupled with capillary electrophoresis (CE) with DAD detection for the determination of carbohydrates has been developed. The chromophore in the 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent is replaced by naphthyl functional group, which results in a reagent with very high molar absorptivity (epsilon(251nm) = 5.58 x 10(4) L mol(-1) cm(-1)). This pen-nits NMP-labeled carbohydrates to be detected with UV absorbance in standard 50-mu m-i.d. fused silica capillaries by zone electrophoresis. in this mode, nanomolar concentrations of detection limits are obtained. The method for the derivatization. of carbohydrates with NMP is simplified. The derivatization reaction is rapid and mild in the presence of ammonia catalyst without further transfer steps. Nine monosaccharide derivatives such as mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be detected in CE mode. Good reproducibility can be obtained with relative standard deviation (R.S.D.) values of the migration times and peak area, respectively, from 0.44 to 0.48 and from 3.2 to 4.8. Furthermore, the developed method has been successfully applied to the analysis of carbohydrates in the hydrolyzed rape bee pollen samples. (C) 2008 Published by Elsevier B.V.
关键词Capillary Electrophoresis (Ce) Carbohydrates 1-(2-naphthyl)-3-methyl-5-pyrazolone (Nmp) Derivatization
WOS标题词Science & Technology ; Physical Sciences
学科领域生物科学
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; DESORPTION/IONIZATION MASS-SPECTROMETRY ; FAST-ATOM-BOMBARDMENT ; 1-PHENYL-3-METHYL-5-PYRAZOLONE DERIVATIVES ; STRUCTURAL CHARACTERIZATION ; SUGAR CHAINS ; OLIGOSACCHARIDES ; ACID ; SEPARATION ; GLYCOPROTEINS
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000253560800008
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1234
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714
You, Jinmao,Sheng, Xiao,Ding, Chenxu,et al. Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)[J]. ANALYTICA CHIMICA ACTA,2008,609(1):66-75.
APA You, Jinmao.,Sheng, Xiao.,Ding, Chenxu.,Sun, Zhiwei.,Suo, Yourui.,...&Li, Win.(2008).Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV).ANALYTICA CHIMICA ACTA,609(1),66-75.
MLA You, Jinmao,et al."Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV)".ANALYTICA CHIMICA ACTA 609.1(2008):66-75.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Detection of carbohy(662KB) 开放获取使用许可浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[You, Jinmao]的文章
[Sheng, Xiao]的文章
[Ding, Chenxu]的文章
百度学术
百度学术中相似的文章
[You, Jinmao]的文章
[Sheng, Xiao]的文章
[Ding, Chenxu]的文章
必应学术
必应学术中相似的文章
[You, Jinmao]的文章
[Sheng, Xiao]的文章
[Ding, Chenxu]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Detection of carbohydrates using new labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone by capillary zone electrophoresis with absorbance (UV).pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。