CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides

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	CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides
	Ding, Chenxu 1,3; Sun, Xuejun 2; Zhao, Xianen 1,4; Zhao, Wenchen 1,2; Li, Yulin 1; Wang, Honglun 1; Suo, Yourui 1; You, Jinmao 1,2
	2008-02-01
发表期刊	CHROMATOGRAPHIA
卷号	67 期号:3-4 页码:245-251
文章类型	Article
摘要	A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column.; A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column.
关键词	Capillary Electrophoresis 2-(9-carbazole)Ethyl Chloroformate (Ceoc-cl) Oligopeptides Derivatization
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域	生物科学
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ZONE-ELECTROPHORESIS ; AMINO-ACID-ANALYSIS ; MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; INDUCED FLUORESCENCE DETECTION ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; NAPHTHALENE-2,3-DICARBOXALDEHYDE DERIVATIZATION ; 2-(9-ANTHRYL)ETHYL CHLOROFORMATE ; PRECOLUMN DERIVATIZATION ; MASS-SPECTROMETRY
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000252806200007
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1238
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Qinghai Nationalities Inst, Dept Pharm, Xining 810007, Qinghai, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ding, Chenxu,Sun, Xuejun,Zhao, Xianen,et al. CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides[J]. CHROMATOGRAPHIA,2008,67(3-4):245-251.
APA	Ding, Chenxu.,Sun, Xuejun.,Zhao, Xianen.,Zhao, Wenchen.,Li, Yulin.,...&You, Jinmao.(2008).CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.CHROMATOGRAPHIA,67(3-4),245-251.
MLA	Ding, Chenxu,et al."CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides".CHROMATOGRAPHIA 67.3-4(2008):245-251.

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