Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau | |
| Zheng, Wei; Wang, Liuyang; Meng, Lihua; Liu, Jianquan | |
| 2008-02-01 | |
| 发表期刊 | GENETICA
 |
| 卷号 | 132期号:2页码:123-129 |
| 文章类型 | Article |
| 摘要 | We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of H-t and H-sp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, H-e and H-pop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei's coefficient of differentiation (G(ST)) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species.; We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of H-t and H-sp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, H-e and H-pop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei's coefficient of differentiation (G(ST)) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species. |
| 关键词 | Anisodus Tanguticus Rapds Genetic Diversity Conservation The Qiughai-tibetan Plateau |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科领域 | 遗传学 |
| 关键词[WOS] | BUFFALOGRASS BUCHLOE-DACTYLOIDES ; AMPLIFIED POLYMORPHIC DNA ; MOLECULAR PHYLOGENY ; RAPD VARIATION ; POPULATIONS ; DIVERSITY ; MARKERS ; CONSERVATION ; PHYLOGEOGRAPHY ; ASTERACEAE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000251308200002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1247 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Qinghai Tibetan Plateau Ecosyst & Biol Ev, Xining 810001, Qinghai, Peoples R China 2.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Wei,Wang, Liuyang,Meng, Lihua,et al. Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau[J]. GENETICA,2008,132(2):123-129. |
| APA | Zheng, Wei,Wang, Liuyang,Meng, Lihua,&Liu, Jianquan.(2008).Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau.GENETICA,132(2),123-129. |
| MLA | Zheng, Wei,et al."Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau".GENETICA 132.2(2008):123-129. |
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