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Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection
Wang, Honglun; Suo, Yourui; Wang, Xiaoyan; Li, Yulin; You, Jinmao; Zhao, Xianen
2007-09-15
发表期刊SEPARATION AND PURIFICATION TECHNOLOGY
卷号56期号:3页码:371-377
文章类型Article
摘要The seed oil from Nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. The extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. The free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as a labeling regent, followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (APCI in positive-ion mode). HPLC analysis shows that the main compositions of the seed oil samples were free fatty acids (FFAs) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. The assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/L. Excellent linear responses were observed, with correlation coefficients greater than 0.999. The facile BDETS derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical CO2 extraction. The established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (C) 2007 Elsevier B.V. All rights reserved.; The seed oil from Nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. The extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. The free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as a labeling regent, followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (APCI in positive-ion mode). HPLC analysis shows that the main compositions of the seed oil samples were free fatty acids (FFAs) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. The assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/L. Excellent linear responses were observed, with correlation coefficients greater than 0.999. The facile BDETS derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical CO2 extraction. The established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (C) 2007 Elsevier B.V. All rights reserved.
关键词Supercritical Carbon Dioxide Extraction Nitraria Tangutorum Bohr. Seed Oil Free Fatty Acids Hplc/apci/ms
WOS标题词Science & Technology ; Technology
学科领域生物科学
关键词[WOS]CO2 EXTRACTION ; SIMULATION
收录类别SCI
语种英语
WOS研究方向Engineering
WOS类目Engineering, Chemical
WOS记录号WOS:000249250900018
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1257
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,et al. Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2007,56(3):371-377.
APA Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,Li, Yulin,You, Jinmao,&Zhao, Xianen.(2007).Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection.SEPARATION AND PURIFICATION TECHNOLOGY,56(3),371-377.
MLA Wang, Honglun,et al."Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection".SEPARATION AND PURIFICATION TECHNOLOGY 56.3(2007):371-377.
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