NWIPB OpenIR
Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection
Wang, Honglun; Suo, Yourui; Wang, Xiaoyan; Li, Yulin; You, Jinmao; Zhao, Xianen
2007-09-15
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
Volume56Issue:3Pages:371-377
SubtypeArticle
AbstractThe seed oil from Nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. The extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. The free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as a labeling regent, followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (APCI in positive-ion mode). HPLC analysis shows that the main compositions of the seed oil samples were free fatty acids (FFAs) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. The assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/L. Excellent linear responses were observed, with correlation coefficients greater than 0.999. The facile BDETS derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical CO2 extraction. The established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (C) 2007 Elsevier B.V. All rights reserved.; The seed oil from Nitraria tangutorum samples was obtained by supercritical carbon dioxide extraction methods. The extraction parameters for this methodology, including pressure, temperature, particle size and extraction time, were optimized. The free fatty acids in the seed oil were separated with a pre-column derivation method and 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as a labeling regent, followed by high-performance liquid chromatography (HPLC) with fluorescence detection. The target compounds were identified by mass spectrometry with atmospheric pressure chemical ionization (APCI in positive-ion mode). HPLC analysis shows that the main compositions of the seed oil samples were free fatty acids (FFAs) in high to low concentrations as follows: linoleic acid, oleic acid, hexadecanoic acid and octadecanoic acid. The assay detection limits (at signal-to-noise of 3:1) were 3.378-6.572 nmol/L. Excellent linear responses were observed, with correlation coefficients greater than 0.999. The facile BDETS derivatization coupled with mass spectrometry detection allowed the development of a highly sensitive method for analyzing free fatty acids in seed oil by supercritical CO2 extraction. The established method is highly efficient for seed oil extraction and extremely sensitive for fatty acid profile determination. (C) 2007 Elsevier B.V. All rights reserved.
KeywordSupercritical Carbon Dioxide Extraction Nitraria Tangutorum Bohr. Seed Oil Free Fatty Acids Hplc/apci/ms
WOS HeadingsScience & Technology ; Technology
Subject Area生物科学
WOS KeywordCO2 EXTRACTION ; SIMULATION
Indexed BySCI
Language英语
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000249250900018
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1257
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,et al. Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2007,56(3):371-377.
APA Wang, Honglun,Suo, Yourui,Wang, Xiaoyan,Li, Yulin,You, Jinmao,&Zhao, Xianen.(2007).Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection.SEPARATION AND PURIFICATION TECHNOLOGY,56(3),371-377.
MLA Wang, Honglun,et al."Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC/APC/IMS with fluorescence detection".SEPARATION AND PURIFICATION TECHNOLOGY 56.3(2007):371-377.
Files in This Item: Download All
File Name/Size DocType Version Access License
Extraction of Nitrar(580KB) 开放获取LicenseView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Honglun]'s Articles
[Suo, Yourui]'s Articles
[Wang, Xiaoyan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Honglun]'s Articles
[Suo, Yourui]'s Articles
[Wang, Xiaoyan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Honglun]'s Articles
[Suo, Yourui]'s Articles
[Wang, Xiaoyan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Extraction of Nitraria tangutorum seed oil by supercritical carbon dioxide and determination of free fatty acids by HPLC APC IMS with fluorescence detection.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.