Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS | |
| Zhao, Xianen; Wang, Honglun; You, Jinmao; Suo, Yourui | |
| 2007-08-01 | |
| 发表期刊 | CHROMATOGRAPHIA
 |
| 卷号 | 66期号:3-4页码:197-206 |
| 文章类型 | Article |
| 摘要 | A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(ptoluenesulfonate)-ethyll-2-phenylimidazole-[4,5-f-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. Identification of FFA derivatives was carried out by HPLC-MS with atmospheric pressure chemical ionization (APCI) in positive ion mode. Gradient elution on an Agilent Eclipse XDB-C-8 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 200 mg of bryophyte plants and soil samples. Facile TSPP derivatization coupled with HPLC-APCI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of FFAs from biological and natural environmental samples.; A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(ptoluenesulfonate)-ethyll-2-phenylimidazole-[4,5-f-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. Identification of FFA derivatives was carried out by HPLC-MS with atmospheric pressure chemical ionization (APCI) in positive ion mode. Gradient elution on an Agilent Eclipse XDB-C-8 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 200 mg of bryophyte plants and soil samples. Facile TSPP derivatization coupled with HPLC-APCI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of FFAs from biological and natural environmental samples. |
| 关键词 | Column Liquid Chromatography-mass Spectrometry Derivatization Fatty Acids Bryophyte 1-[2-(P-toluenesulfonate)-ethyl]-2-phenylimidazole-[4 5-f]-9 10 Phenanthrene (Tspp) |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科领域 | 生物化学 |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; LABELING REAGENT ; PRECOLUMN DERIVATIZATION ; TRIFLUOROMETHANESULFONATE ; DERIVATIVES ; LIVERWORTS ; JAPANESE ; 9-ANTHRYLDIAZOMETHANE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000249232900009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1258 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xianen,Wang, Honglun,You, Jinmao,et al. Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS[J]. CHROMATOGRAPHIA,2007,66(3-4):197-206. |
| APA | Zhao, Xianen,Wang, Honglun,You, Jinmao,&Suo, Yourui.(2007).Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS.CHROMATOGRAPHIA,66(3-4),197-206. |
| MLA | Zhao, Xianen,et al."Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS".CHROMATOGRAPHIA 66.3-4(2007):197-206. |
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