Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS

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	Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS
	Wang, Li ; Xiao, Hongbin ; Liang, Xinmiao ; Wei, Lixin
	2007-07-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	30 期号:10 页码:1488-1495
文章类型	Article
摘要	An HPLC-UV-MS method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in Gastrodia elata was developed, which was based on their UV and MS characteristics summarized through a series of homemade reference standard experiments. Phenolics showed characteristic UV lambda(max) at 267 nm, [M + NH4](+) base peak in positive mode and [M - H](-) base peak in negative mode while nucleosides exhibited UV lambda(max) at 255 nm, [M + H](+), [M - H + 2H(2)O](-) or [M - H + CH3COOH](-). Phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 U) and the combined loss of H2O and CO2 from the citric acid unit under negative MS/MS conditions whereas nucleosides simply lost the ribose (- 132 U) under positive MS/MS conditions. According to these characteristics, a special pattern under MS/MS conditions and reported compound data for G. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. Among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from G. elata.; An HPLC-UV-MS method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in Gastrodia elata was developed, which was based on their UV and MS characteristics summarized through a series of homemade reference standard experiments. Phenolics showed characteristic UV lambda(max) at 267 nm, [M + NH4](+) base peak in positive mode and [M - H](-) base peak in negative mode while nucleosides exhibited UV lambda(max) at 255 nm, [M + H](+), [M - H + 2H(2)O](-) or [M - H + CH3COOH](-). Phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 U) and the combined loss of H2O and CO2 from the citric acid unit under negative MS/MS conditions whereas nucleosides simply lost the ribose (- 132 U) under positive MS/MS conditions. According to these characteristics, a special pattern under MS/MS conditions and reported compound data for G. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. Among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from G. elata.
关键词	Gastrodia Elata Lc-ms Nucleoside Phenolic
WOS标题词	Science & Technology ; Physical Sciences
学科领域	生物科学
关键词[WOS]	P-HYDROXYBENZYL ALCOHOL ; CAPILLARY-ELECTROPHORESIS ; MASS-SPECTROMETRY ; PARISHIN-B ; BLUME ; CHROMATOGRAPHY ; INHIBITION ; RHIZOMA
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000248240000013
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1262
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Res Ctr Tibetan Med, NW Plateau Inst Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Xiao, Hongbin,Liang, Xinmiao,et al. Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1488-1495.
APA	Wang, Li,Xiao, Hongbin,Liang, Xinmiao,&Wei, Lixin.(2007).Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS.JOURNAL OF SEPARATION SCIENCE,30(10),1488-1495.
MLA	Wang, Li,et al."Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS".JOURNAL OF SEPARATION SCIENCE 30.10(2007):1488-1495.

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