Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers

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	Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers
	Peng, Xiao-Li ; Zhao, Chang-Ming ; Wu, Gui-Li ; Liu, Jian-Quan
	2007-07-01
发表期刊	TREES-STRUCTURE AND FUNCTION
卷号	21 期号:4 页码:457-464
文章类型	Article
摘要	Repeated cycles of retreat and recolonization during the Quaternary ice ages are thought to have greatly influenced current species distributions and their genetic diversity. It remains unclear how this climatic oscillation has affected the distribution of genetic diversity between populations of wind-pollinated conifers in the Qinghai-Tibetan region. In this study, we investigated the within-species genetic diversity and phylogenetic relationships of Picea likiangensis, a dominant forest species in this region using polymorphic DNA (RAPD) markers. Our results suggest that this species has high overall genetic diversity, with 85.42% of loci being polymorphic and an average expected heterozygosity (H (E)) of 0.239. However, there were relatively low levels of polymorphism at population levels and the differences between populations were not significant, with percentages of polymorphic bands (PPB) ranging from 46.88 to 69.76%, Nei's gene diversity (H (E)) from 0.179 to 0.289 and Shannon's indices (Hpop) from 0.267 to 0.421. In accordance with our proposed hypothesis, a high level of genetic differentiation among populations was detected based on Nei's genetic diversity (G (ST) = 0.256) and AMOVA analysis (Phi (st) = 0.236). Gene flow between populations was found to be limited (Nm = 1.4532) and far lower than reported for other conifer species with wide distribution ranges from other regions. No clusters corresponding to three morphological varieties found in the south, north and west, respectively, were detected in either UPGMA or PCO analyses. Our results suggest that this species may have had different refugia during the glacial stages in the southern region and that the northern variety may have multiple origins from these different refugia.; Repeated cycles of retreat and recolonization during the Quaternary ice ages are thought to have greatly influenced current species distributions and their genetic diversity. It remains unclear how this climatic oscillation has affected the distribution of genetic diversity between populations of wind-pollinated conifers in the Qinghai-Tibetan region. In this study, we investigated the within-species genetic diversity and phylogenetic relationships of Picea likiangensis, a dominant forest species in this region using polymorphic DNA (RAPD) markers. Our results suggest that this species has high overall genetic diversity, with 85.42% of loci being polymorphic and an average expected heterozygosity (H (E)) of 0.239. However, there were relatively low levels of polymorphism at population levels and the differences between populations were not significant, with percentages of polymorphic bands (PPB) ranging from 46.88 to 69.76%, Nei's gene diversity (H (E)) from 0.179 to 0.289 and Shannon's indices (Hpop) from 0.267 to 0.421. In accordance with our proposed hypothesis, a high level of genetic differentiation among populations was detected based on Nei's genetic diversity (G (ST) = 0.256) and AMOVA analysis (Phi (st) = 0.236). Gene flow between populations was found to be limited (Nm = 1.4532) and far lower than reported for other conifer species with wide distribution ranges from other regions. No clusters corresponding to three morphological varieties found in the south, north and west, respectively, were detected in either UPGMA or PCO analyses. Our results suggest that this species may have had different refugia during the glacial stages in the southern region and that the northern variety may have multiple origins from these different refugia.
关键词	Picea Likiangensis Genetic Diversity Rapd Morphological Differentiation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SOUTH-AMERICAN CONIFER ; FOREST TREES ; POPULATIONS ; DIVERSITY ; ALLOZYME ; EVOLUTION ; DIFFERENTIATION ; CONSERVATION ; CUPRESSACEAE ; CALIFORNIA
收录类别	SCI
语种	英语
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000247659000007
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1265
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xiao-Li,Zhao, Chang-Ming,Wu, Gui-Li,et al. Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers[J]. TREES-STRUCTURE AND FUNCTION,2007,21(4):457-464.
APA	Peng, Xiao-Li,Zhao, Chang-Ming,Wu, Gui-Li,&Liu, Jian-Quan.(2007).Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers.TREES-STRUCTURE AND FUNCTION,21(4),457-464.
MLA	Peng, Xiao-Li,et al."Genetic variation and phylogeographic history of Picea likiangensis revealed by RAPD markers".TREES-STRUCTURE AND FUNCTION 21.4(2007):457-464.

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