Separation and determination of the major active components in Tibetan folk medicinal species Swertia franchetiana by HPLC with DAD

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	Separation and determination of the major active components in Tibetan folk medicinal species Swertia franchetiana by HPLC with DAD
	Li, Yulin ; Wang, Lingyun ; Wang, Xiaoyan ; Suo, Yourui ; Chen, Guichen ; Hu, Fengzu ; Du, Yuzhi ; You, Jinmao
	2007
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号	30 期号:9-12 页码:1687-1696
文章类型	Article
摘要	A sensitive and specific reversed-phase high performance liquid chromatography (RP-HPLC) method with diode array detection (DAD) was established for the quantitative determination of the nine active components, namely, swertiamarin (SWM, 1), mangiferin (MA, 2), gentipicroside (GE, 3), sweroside (SWO, 4), isoorientin (IS, 5), swertisin (SWS, 6), swertianolin (SWN, 7), 7-O-[alpha-L-rhamnopyranosyl-1 -> 2)-beta-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone (RX, 8), and bellidifolin (BE, 9) used as the external standard, in Tibetan folk medicinal species Swertia franchetiana. Based on the baseline chromatographic separation of most components from the methanolic extract of Swertia franchetiana on a reversed-phase Eclipse XDB-C8 column with water-acetonitrile-formic acid as mobile phase, the nine components were identified by comparison with standard samples and qualified by using the external standard method with DAD at 254 nm. The correlation coefficients of all the calibration curves were found to be higher than 0.9980. The relative standard deviations (RSDs) of the peak areas and retention times for the nine standards were less than 2.07% and 2.86%, respectively.; A sensitive and specific reversed-phase high performance liquid chromatography (RP-HPLC) method with diode array detection (DAD) was established for the quantitative determination of the nine active components, namely, swertiamarin (SWM, 1), mangiferin (MA, 2), gentipicroside (GE, 3), sweroside (SWO, 4), isoorientin (IS, 5), swertisin (SWS, 6), swertianolin (SWN, 7), 7-O-[alpha-L-rhamnopyranosyl-1 -> 2)-beta-D-xylopyranosyl]-1,8-dihydroxy-3-methoxyxanthone (RX, 8), and bellidifolin (BE, 9) used as the external standard, in Tibetan folk medicinal species Swertia franchetiana. Based on the baseline chromatographic separation of most components from the methanolic extract of Swertia franchetiana on a reversed-phase Eclipse XDB-C8 column with water-acetonitrile-formic acid as mobile phase, the nine components were identified by comparison with standard samples and qualified by using the external standard method with DAD at 254 nm. The correlation coefficients of all the calibration curves were found to be higher than 0.9980. The relative standard deviations (RSDs) of the peak areas and retention times for the nine standards were less than 2.07% and 2.86%, respectively.
关键词	Swertiamarin Gentipicroside Sweroside Swertianolin Bellidifolin Swertia Franchetiana
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域	生物化学
关键词[WOS]	CENTAURIUM-ERYTHRAEA ; GLYCOSIDES ; BIOACTIVITY ; XANTHONES
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000246884000033
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1272
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qufu Normal Univ, Dept Chem, Qufu, Peoples R China
推荐引用方式 GB/T 7714	Li, Yulin,Wang, Lingyun,Wang, Xiaoyan,et al. Separation and determination of the major active components in Tibetan folk medicinal species Swertia franchetiana by HPLC with DAD[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2007,30(9-12):1687-1696.
APA	Li, Yulin.,Wang, Lingyun.,Wang, Xiaoyan.,Suo, Yourui.,Chen, Guichen.,...&You, Jinmao.(2007).Separation and determination of the major active components in Tibetan folk medicinal species Swertia franchetiana by HPLC with DAD.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,30(9-12),1687-1696.
MLA	Li, Yulin,et al."Separation and determination of the major active components in Tibetan folk medicinal species Swertia franchetiana by HPLC with DAD".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 30.9-12(2007):1687-1696.

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