Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS | |
You, Jinmao; Zhao, Xianen; Suo, Yourui; Li, Yulin; Wang, Honglun; Chen, Guichen | |
2007-04-01 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
卷号 | 848期号:2页码:283-291 |
文章类型 | Article |
摘要 | A sensitive method for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (TSPP) as tagging reagent with fluorescence detection and identification with post-column APCI/MS has been developed. The LCFAs in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. TSPP could easily and quickly label LCFAs at 90 degrees C in the presence of K2CO3 catalyst in DMF. Eleven free LCFAs from the extracts of bryophyte plants were sensitively determined. Maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. Separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Eclipse XDB-C-8 column. Calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. Excellent linear responses were observed with coefficients of >0.9996. Good compositional data were obtained from the analysis of the extracted LCFAs containing as little as 0.2 g of bryophyte plant samples. Therefore, the facile TSPP derivatization coupled with HPLC/APCI/MS analysis allowed the development of a highly sensitive method for the quantitation of trace levels of LCFAs from biological and natural environmental samples. (c) 2006 Elsevier B.V. All rights reserved.; A sensitive method for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (TSPP) as tagging reagent with fluorescence detection and identification with post-column APCI/MS has been developed. The LCFAs in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. TSPP could easily and quickly label LCFAs at 90 degrees C in the presence of K2CO3 catalyst in DMF. Eleven free LCFAs from the extracts of bryophyte plants were sensitively determined. Maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. Separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Eclipse XDB-C-8 column. Calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. Excellent linear responses were observed with coefficients of >0.9996. Good compositional data were obtained from the analysis of the extracted LCFAs containing as little as 0.2 g of bryophyte plant samples. Therefore, the facile TSPP derivatization coupled with HPLC/APCI/MS analysis allowed the development of a highly sensitive method for the quantitation of trace levels of LCFAs from biological and natural environmental samples. (c) 2006 Elsevier B.V. All rights reserved. |
关键词 | Derivatization 1-[2-(P-toluenesulfonate)-ethyl]-2-phenylimidazole-[4 Hplc 5-f]-9 Fatty Acids 10-phenanthrene (Tspp) Mass Spectrometry |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
学科领域 | 生物化学 |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; LABELING REAGENT ; PRECOLUMN DERIVATIZATION ; FLUOROMETRIC DETECTION ; DERIVATIVES ; LIVERWORTS ; JAPANESE ; SERUM |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000245762600016 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1282 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China |
推荐引用方式 GB/T 7714 | You, Jinmao,Zhao, Xianen,Suo, Yourui,et al. Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,848(2):283-291. |
APA | You, Jinmao,Zhao, Xianen,Suo, Yourui,Li, Yulin,Wang, Honglun,&Chen, Guichen.(2007).Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,848(2),283-291. |
MLA | You, Jinmao,et al."Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 848.2(2007):283-291. |
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