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Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS
You, Jinmao; Zhao, Xianen; Suo, Yourui; Li, Yulin; Wang, Honglun; Chen, Guichen
2007-04-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume848Issue:2Pages:283-291
SubtypeArticle
AbstractA sensitive method for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (TSPP) as tagging reagent with fluorescence detection and identification with post-column APCI/MS has been developed. The LCFAs in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. TSPP could easily and quickly label LCFAs at 90 degrees C in the presence of K2CO3 catalyst in DMF. Eleven free LCFAs from the extracts of bryophyte plants were sensitively determined. Maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. Separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Eclipse XDB-C-8 column. Calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. Excellent linear responses were observed with coefficients of >0.9996. Good compositional data were obtained from the analysis of the extracted LCFAs containing as little as 0.2 g of bryophyte plant samples. Therefore, the facile TSPP derivatization coupled with HPLC/APCI/MS analysis allowed the development of a highly sensitive method for the quantitation of trace levels of LCFAs from biological and natural environmental samples. (c) 2006 Elsevier B.V. All rights reserved.; A sensitive method for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (TSPP) as tagging reagent with fluorescence detection and identification with post-column APCI/MS has been developed. The LCFAs in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. TSPP could easily and quickly label LCFAs at 90 degrees C in the presence of K2CO3 catalyst in DMF. Eleven free LCFAs from the extracts of bryophyte plants were sensitively determined. Maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. Separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Eclipse XDB-C-8 column. Calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. Excellent linear responses were observed with coefficients of >0.9996. Good compositional data were obtained from the analysis of the extracted LCFAs containing as little as 0.2 g of bryophyte plant samples. Therefore, the facile TSPP derivatization coupled with HPLC/APCI/MS analysis allowed the development of a highly sensitive method for the quantitation of trace levels of LCFAs from biological and natural environmental samples. (c) 2006 Elsevier B.V. All rights reserved.
KeywordDerivatization 1-[2-(P-toluenesulfonate)-ethyl]-2-phenylimidazole-[4 Hplc 5-f]-9 Fatty Acids 10-phenanthrene (Tspp) Mass Spectrometry
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Subject Area生物化学
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; LABELING REAGENT ; PRECOLUMN DERIVATIZATION ; FLUOROMETRIC DETECTION ; DERIVATIVES ; LIVERWORTS ; JAPANESE ; SERUM
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000245762600016
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1282
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China
Recommended Citation
GB/T 7714
You, Jinmao,Zhao, Xianen,Suo, Yourui,et al. Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,848(2):283-291.
APA You, Jinmao,Zhao, Xianen,Suo, Yourui,Li, Yulin,Wang, Honglun,&Chen, Guichen.(2007).Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,848(2),283-291.
MLA You, Jinmao,et al."Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 848.2(2007):283-291.
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