Knowledge Management System of Northwest Institute of Plateau Biology, CAS
ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae) | |
Xia, Tao; Chen, Shilong; Chen, Shenyun; Zhang, Defang; Zhang, Dejun; Gao, Qingbo; Ge, Xuejun | |
2007-04-01 | |
发表期刊 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY |
卷号 | 35期号:4页码:209-214 |
文章类型 | Article |
摘要 | Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved.; Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved. |
关键词 | Rhodiola Chrysanthemifolia Issrs Genetic Diversity Qinghai-tibet Plateau |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科领域 | 分子生物学 |
关键词[WOS] | RAPD VARIATION ; POPULATION |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
WOS记录号 | WOS:000245696000004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1284 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol Evolut & Adaptat, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, S China Inst Bot, Guangzhou 510650, Peoples R China |
推荐引用方式 GB/T 7714 | Xia, Tao,Chen, Shilong,Chen, Shenyun,et al. ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2007,35(4):209-214. |
APA | Xia, Tao.,Chen, Shilong.,Chen, Shenyun.,Zhang, Defang.,Zhang, Dejun.,...&Ge, Xuejun.(2007).ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae).BIOCHEMICAL SYSTEMATICS AND ECOLOGY,35(4),209-214. |
MLA | Xia, Tao,et al."ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 35.4(2007):209-214. |
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