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ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)
Xia, Tao; Chen, Shilong; Chen, Shenyun; Zhang, Defang; Zhang, Dejun; Gao, Qingbo; Ge, Xuejun
2007-04-01
发表期刊BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷号35期号:4页码:209-214
文章类型Article
摘要Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved.; Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. (c) 2006 Elsevier Ltd. All rights reserved.
关键词Rhodiola Chrysanthemifolia Issrs Genetic Diversity Qinghai-tibet Plateau
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科领域分子生物学
关键词[WOS]RAPD VARIATION ; POPULATION
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号WOS:000245696000004
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1284
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol Evolut & Adaptat, Xining 810001, Qinghai, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, S China Inst Bot, Guangzhou 510650, Peoples R China
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Xia, Tao,Chen, Shilong,Chen, Shenyun,et al. ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2007,35(4):209-214.
APA Xia, Tao.,Chen, Shilong.,Chen, Shenyun.,Zhang, Defang.,Zhang, Dejun.,...&Ge, Xuejun.(2007).ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae).BIOCHEMICAL SYSTEMATICS AND ECOLOGY,35(4),209-214.
MLA Xia, Tao,et al."ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic, Rhodiola chrysanthemifolia (Crassulaceae)".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 35.4(2007):209-214.
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