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Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection
You, Jinmao; Zhu, Fang; Zhao, Wenchen; Zhao, Xian'en; Suo, Yourui; Liu, Sujuan
2007-03-01
Source PublicationEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Volume109Issue:3Pages:225-236
SubtypeArticle
AbstractA sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids.; A sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids.
KeywordRape Bee Pollen Fatty Acids Hplc 2-(2-(Anthracen-10-yl)-1h-naphtho[2 Fluorescence Detection. 3-d]Imidazol-1-yl) Ethyl-p-toluenesulfonate (Anits)
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Subject Area生物科学
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; DERIVATIZATION REAGENT ; LABELING REAGENT ; FLUOROMETRIC DETECTION ; TRIFLUOROMETHANESULFONATE ; 9-ANTHRYLDIAZOMETHANE ; DERIVATIVES ; PROSTAGLANDINS ; SERUM
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology ; Nutrition & Dietetics
WOS SubjectFood Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000245200500006
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1287
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China
Recommended Citation
GB/T 7714
You, Jinmao,Zhu, Fang,Zhao, Wenchen,et al. Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2007,109(3):225-236.
APA You, Jinmao,Zhu, Fang,Zhao, Wenchen,Zhao, Xian'en,Suo, Yourui,&Liu, Sujuan.(2007).Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,109(3),225-236.
MLA You, Jinmao,et al."Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 109.3(2007):225-236.
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