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Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE
Wang, Honglun; Wang, Xiaoyan; You, Jinmao; Suo, Yourui; Zhao, Xianen; Li, Yulin; Ding, Chenxu
2007-02-01
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号30期号:3页码:363-376
文章类型Article
摘要This paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA) in Nitraria tangutorum Bobr seed by HPLC-APCI-MS and CE (capillary electrophoresis) methods. The final optimized chromatographic conditions were investigated in a reversed-phase Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m). A seventeen-minute gradient elution, (A: aqueous acetonitrile 20% (v/v); B: aqueous acetonitrile 60% (v/v); C: pure acetonitrile 100%) at a flow rate of 1.0 mL/min was selected for the separation of three natural products with diode array detection (DAD) at 220 nm. A CE experiment was carried out in a fused silica capillary with 32 mmol/L boric acid (pH 10), 32 mmol/L SDS and acetonitrile (10.0%, v/v). The applied potential and temperature was, respectively, set at 19 kV and 25 degrees C. After development, the validation was performed in parallel for HPLC and CE, with the same standards and sample to avoid differences due to the manipulation. The validation parameters of both techniques were adequate for the intended purpose.; This paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA) in Nitraria tangutorum Bobr seed by HPLC-APCI-MS and CE (capillary electrophoresis) methods. The final optimized chromatographic conditions were investigated in a reversed-phase Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m). A seventeen-minute gradient elution, (A: aqueous acetonitrile 20% (v/v); B: aqueous acetonitrile 60% (v/v); C: pure acetonitrile 100%) at a flow rate of 1.0 mL/min was selected for the separation of three natural products with diode array detection (DAD) at 220 nm. A CE experiment was carried out in a fused silica capillary with 32 mmol/L boric acid (pH 10), 32 mmol/L SDS and acetonitrile (10.0%, v/v). The applied potential and temperature was, respectively, set at 19 kV and 25 degrees C. After development, the validation was performed in parallel for HPLC and CE, with the same standards and sample to avoid differences due to the manipulation. The validation parameters of both techniques were adequate for the intended purpose.
关键词Allantoin Quercetin Nitraria Tangutorum Bobr 1-methyl-1 2 Capillary Electrophoresis 3 High Performance Liquid Chromatography 4-tetrahydro-beta-carboline-3-car-boxylicb Acid Atmospheric Pressure Chemical Ionization Mass Spectrometry
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域生物化学
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000243121900004
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1302
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Platueau Inst Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wang, Honglun,Wang, Xiaoyan,You, Jinmao,et al. Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2007,30(3):363-376.
APA Wang, Honglun.,Wang, Xiaoyan.,You, Jinmao.,Suo, Yourui.,Zhao, Xianen.,...&Ding, Chenxu.(2007).Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,30(3),363-376.
MLA Wang, Honglun,et al."Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 30.3(2007):363-376.
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