NWIPB OpenIR
Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE
Wang, Honglun; Wang, Xiaoyan; You, Jinmao; Suo, Yourui; Zhao, Xianen; Li, Yulin; Ding, Chenxu
2007-02-01
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume30Issue:3Pages:363-376
SubtypeArticle
AbstractThis paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA) in Nitraria tangutorum Bobr seed by HPLC-APCI-MS and CE (capillary electrophoresis) methods. The final optimized chromatographic conditions were investigated in a reversed-phase Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m). A seventeen-minute gradient elution, (A: aqueous acetonitrile 20% (v/v); B: aqueous acetonitrile 60% (v/v); C: pure acetonitrile 100%) at a flow rate of 1.0 mL/min was selected for the separation of three natural products with diode array detection (DAD) at 220 nm. A CE experiment was carried out in a fused silica capillary with 32 mmol/L boric acid (pH 10), 32 mmol/L SDS and acetonitrile (10.0%, v/v). The applied potential and temperature was, respectively, set at 19 kV and 25 degrees C. After development, the validation was performed in parallel for HPLC and CE, with the same standards and sample to avoid differences due to the manipulation. The validation parameters of both techniques were adequate for the intended purpose.; This paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA) in Nitraria tangutorum Bobr seed by HPLC-APCI-MS and CE (capillary electrophoresis) methods. The final optimized chromatographic conditions were investigated in a reversed-phase Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m). A seventeen-minute gradient elution, (A: aqueous acetonitrile 20% (v/v); B: aqueous acetonitrile 60% (v/v); C: pure acetonitrile 100%) at a flow rate of 1.0 mL/min was selected for the separation of three natural products with diode array detection (DAD) at 220 nm. A CE experiment was carried out in a fused silica capillary with 32 mmol/L boric acid (pH 10), 32 mmol/L SDS and acetonitrile (10.0%, v/v). The applied potential and temperature was, respectively, set at 19 kV and 25 degrees C. After development, the validation was performed in parallel for HPLC and CE, with the same standards and sample to avoid differences due to the manipulation. The validation parameters of both techniques were adequate for the intended purpose.
KeywordAllantoin Quercetin Nitraria Tangutorum Bobr 1-methyl-1 2 Capillary Electrophoresis 3 High Performance Liquid Chromatography 4-tetrahydro-beta-carboline-3-car-boxylicb Acid Atmospheric Pressure Chemical Ionization Mass Spectrometry
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Subject Area生物化学
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000243121900004
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1302
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Platueau Inst Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang, Honglun,Wang, Xiaoyan,You, Jinmao,et al. Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2007,30(3):363-376.
APA Wang, Honglun.,Wang, Xiaoyan.,You, Jinmao.,Suo, Yourui.,Zhao, Xianen.,...&Ding, Chenxu.(2007).Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,30(3),363-376.
MLA Wang, Honglun,et al."Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 30.3(2007):363-376.
Files in This Item: Download All
File Name/Size DocType Version Access License
2007Comparative Anal(150KB) 开放获取LicenseView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Honglun]'s Articles
[Wang, Xiaoyan]'s Articles
[You, Jinmao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Honglun]'s Articles
[Wang, Xiaoyan]'s Articles
[You, Jinmao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Honglun]'s Articles
[Wang, Xiaoyan]'s Articles
[You, Jinmao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2007Comparative Analysis of Allantoin,Quercetin, and.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.