Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii

NWIPB OpenIR

	Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii
	Xue, Chun-Ying ; Li, De-Zhu ; Lu, Jin-Mei ; Yang, Jun-Bo ; Liu, Jian-Quan
	2006-10-01
发表期刊	PLANTA MEDICA
卷号	72 期号:13 页码:1223-1226
文章类型	Article
摘要	Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.; Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.
关键词	Tibetan Folk Medicine Swertia Mussotii Gentianaceae Dna Sequences Allele-specific Diagnostic Pcr Authentication
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
学科领域	植物学
关键词[WOS]	DENDROBIUM-OFFICINALE ; RIBOSOMAL DNA ; NUCLEAR ; REGION ; SPACER
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000241876600009
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1307
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Lab Plant Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,et al. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii[J]. PLANTA MEDICA,2006,72(13):1223-1226.
APA	Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,Yang, Jun-Bo,&Liu, Jian-Quan.(2006).Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii.PLANTA MEDICA,72(13),1223-1226.
MLA	Xue, Chun-Ying,et al."Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii".PLANTA MEDICA 72.13(2006):1223-1226.