Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay

doi:10.1016/j.indcrop.2015.01.063

NWIPB OpenIR

	Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay
	Geng, Dandan 1,2; Chi, Xiaofeng 1; Dong, Qi 1; Hu, Fengzu 1
	2015-05-01
发表期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
卷号	67 页码:492-497
文章类型	Article
摘要	Limonium aureum (Linn.) is a traditional Chinese medicine with strong antioxidant activity. To search for antioxidants in L. aureum, an optimized, on-line high performance liquid chromatography (HPLC) method with DPPH (1,1-diphenyl-2-picrylhydrazyl) for the detection of radical scavenging ability was developed. Response surface methodology (RSM) was used to determine the best combination of experimental conditions, such as DPPH concentration, DPPH velocity and the reaction tank length. The optimized method uses a DPPH concentration of 25.0 mu g/mL, DPPH velocity of 0.45 mL/min and a 15.0 m long reaction tank. The highly sensitive, optimized method can not only be used for the detection of antioxidants in plants, but can also be coupled with mass spectrometry (MS) to obtain the mass-to-charge ratios of chemical species corresponding to the different peaks in the HPLC profiles. Seven antioxidants were identified in L. aureum using the optimized method, including myricetin-3-O-beta-D-(6 ''-O-galloyl)-glucopyranoside, myricetin-3-O-glucoside, myricitrin, eriodictyol-7-O-glucoside, myricetin, eriodictyol and homoeridictyol. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Limonium Aureum (Linn.) Antioxidant On-line Hplc-dpph Response Surface Methodology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.indcrop.2015.01.063
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; ANTHOCYANINS ; CAPACITY ; DISEASES ; FRUITS ; PHASE
收录类别	SCI
语种	英语
项目资助者	Equipment Function Development Innovation Programs of the Chinese Academy of Sciences(yg2012086)
WOS研究方向	Agriculture
WOS类目	Agricultural Engineering ; Agronomy
WOS记录号	WOS:000352039600064
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13265
专题	中国科学院西北高原生物研究所
通讯作者	Chi, Xiaofeng
作者单位	1.Chinese Acad Sci, North West Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Geng, Dandan,Chi, Xiaofeng,Dong, Qi,et al. Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay[J]. INDUSTRIAL CROPS AND PRODUCTS,2015,67:492-497.
APA	Geng, Dandan,Chi, Xiaofeng,Dong, Qi,&Hu, Fengzu.(2015).Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay.INDUSTRIAL CROPS AND PRODUCTS,67,492-497.
MLA	Geng, Dandan,et al."Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay".INDUSTRIAL CROPS AND PRODUCTS 67(2015):492-497.