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Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis
Tian, Fei1,2,3; Tong, Chao1,2,3,4; Feng, Chenguang1,2,3,4; Wanghe, Kunyuan1,2,3,4; Zhao, Kai1,2,3
2017-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume70Pages:524-535
SubtypeArticle
AbstractGymnocypris przewalskii is a native cyprinid in the Lake Qinghai of the Qinghai-Tibetan Plateau. G. przewalskii is highly susceptible to the infection of a parasite, Ichthyophthirius multifiliis, in the artificial propagation and breeding. To better understand the host immune reaction to I. multifiliis infection, we characterize the gene expression profiles in the spleen of healthy and I. multifiliis infected G. przewalskii by RNA-seq. Totally, the transcriptomic analysis produces 463,031,110 high quality reads, which are assembled to 213,538 genes with N50 of 1918 bp and the average length of 1205 bp. Of assembled genes, 90.52% are annotated by public databases. The expression analysis shows 744 genes are significantly changed by the infection of I. multifiliis, which are validated by qRT-PCR with the correlation coefficient of 0.896. The differentially expressed genes are classified into 689 GO terms and 230 KEGG pathways, highlighting the promoted innate immunity in I. multifiliis infected G. przewalskii at 2 days post infection. Our results pinpoint that the up-regulated genes are enriched in TLR signaling pathway, inflammatory response and activation of immune cell migration. On the contrary, complement genes are down regulated, indicating the evasion of host complement cascades by I. multifiliis. The repressed genes are also enriched in the pathways related to metabolism and endocrine, suggesting the metabolic disturbance in I. multifiliis treated G. przewalskii. In summary, the present study profiles the gene expression signature of G. przewalskii in the responses to I. multifiliis infection, and improves our understanding on molecular mechanisms of host-parasite interaction in G. przewalskii, which focuses the crucial function of TLRs, cytokines and complement components in the host defense against I. multifiliis. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordGymnocypris Przewalskii Ichthyophthirius Multifiliis Parasite Infection Rna-seq Immune Responses
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.fsi.2017.09.003
WOS KeywordZEBRAFISH DANIO-RERIO ; CARP CYPRINUS-CARPIO ; TOLL-LIKE RECEPTORS ; ONCORHYNCHUS-MYKISS WALBAUM ; IMMUNE-RELEVANT GENES ; INNATE IMMUNITY ; RAINBOW-TROUT ; CHANNEL CATFISH ; EXPRESSION ANALYSIS ; COMPLEMENT EVASION
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(31700325) ; Science Foundation of Qinghai Province(2016-ZJ-941Q) ; Construction Fund for Qinghai Key Laboratories(2017-41-Y23) ; "1000 Talent" programs of Qinghai Province
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000416186800055
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13272
Collection中国科学院西北高原生物研究所
Corresponding AuthorZhao, Kai
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Lab Plateau Fish Evolutionary & Funct Genom, Xining, Qinghai, Peoples R China
3.Qinghai Key Lab Anim Ecol Genom, Xining, Qinghai, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Tian, Fei,Tong, Chao,Feng, Chenguang,et al. Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:524-535.
APA Tian, Fei,Tong, Chao,Feng, Chenguang,Wanghe, Kunyuan,&Zhao, Kai.(2017).Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis.FISH & SHELLFISH IMMUNOLOGY,70,524-535.
MLA Tian, Fei,et al."Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis".FISH & SHELLFISH IMMUNOLOGY 70(2017):524-535.
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