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A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging
Guo, Lan1; Yang, Daoshan1; Xia, Lian1; Qu, Fei1; Dou, Yanan1; Qu, Fengli1; Kong, Rongmei1; You, Jinmao1,2
2017-12-21
发表期刊NEW JOURNAL OF CHEMISTRY
ISSN1144-0546
卷号41期号:24页码:15277-15282
文章类型Article
摘要In this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells.
WOS标题词Science & Technology ; Physical Sciences
DOI10.1039/c7nj02391j
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; LIVING CELLS ; CAPILLARY-ELECTROPHORESIS ; SELECTIVE DETECTION ; METHYLMALONIC ACID ; HUMAN PLASMA ; IN-VIVO ; CYSTEINE ; HOMOCYSTEINE ; GLUTATHIONE
收录类别SCI
语种英语
项目资助者Natural Science Foundation of Shandong Province(ZR2014BM029) ; National Natural Science Foundation of China(21505084 ; 21375076 ; 21475075 ; 21405093)
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000417058800063
出版者ROYAL SOC CHEMISTRY
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/13284
专题中国科学院西北高原生物研究所
通讯作者Xia, Lian
作者单位1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining, Qinghai, Peoples R China
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GB/T 7714
Guo, Lan,Yang, Daoshan,Xia, Lian,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017,41(24):15277-15282.
APA Guo, Lan.,Yang, Daoshan.,Xia, Lian.,Qu, Fei.,Dou, Yanan.,...&You, Jinmao.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY,41(24),15277-15282.
MLA Guo, Lan,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY 41.24(2017):15277-15282.
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