Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging | |
Guo, Lan1; Yang, Daoshan1; Xia, Lian1; Qu, Fei1; Dou, Yanan1; Qu, Fengli1; Kong, Rongmei1; You, Jinmao1,2 | |
2017-12-21 | |
发表期刊 | NEW JOURNAL OF CHEMISTRY |
ISSN | 1144-0546 |
卷号 | 41期号:24页码:15277-15282 |
文章类型 | Article |
摘要 | In this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells. |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1039/c7nj02391j |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; LIVING CELLS ; CAPILLARY-ELECTROPHORESIS ; SELECTIVE DETECTION ; METHYLMALONIC ACID ; HUMAN PLASMA ; IN-VIVO ; CYSTEINE ; HOMOCYSTEINE ; GLUTATHIONE |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Natural Science Foundation of Shandong Province(ZR2014BM029) ; National Natural Science Foundation of China(21505084 ; 21375076 ; 21475075 ; 21405093) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000417058800063 |
出版者 | ROYAL SOC CHEMISTRY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/13284 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Xia, Lian |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Guo, Lan,Yang, Daoshan,Xia, Lian,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017,41(24):15277-15282. |
APA | Guo, Lan.,Yang, Daoshan.,Xia, Lian.,Qu, Fei.,Dou, Yanan.,...&You, Jinmao.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY,41(24),15277-15282. |
MLA | Guo, Lan,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY 41.24(2017):15277-15282. |
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