Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates

doi:10.1186/s13568-017-0497-8

NWIPB OpenIR

	Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates
	Pei, Jinjin 1,2; Jiang, Hai 1; Li, Xinsheng 1; Jin, Wengang 1; Tao, Yanduo 2,3
	2017-12-06
发表期刊	AMB EXPRESS
ISSN	2191-0855
卷号	7
文章类型	Article
摘要	Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs).
关键词	Antimicrobial Peptides Yak Milk Hydrolysates Isolation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1186/s13568-017-0497-8
关键词[WOS]	LISTERIA-MONOCYTOGENES ; ANTIBACTERIAL ACTIVITY ; BACTERIOCIN ; CHROMATOGRAPHY ; PURIFICATION ; SKINS ; BLOOD ; DEER
收录类别	SCI
语种	英语
项目资助者	Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Collaborative innovation center Research Foundation(QBXT-Z(P)-15-28) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Scientists' Foundations of Shaanxi University of Technology(SLGKYQD2014-2-18) ; Research Foundations of Shaanxi University of Technology(SLGQD13-13)
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000417529100001
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13285
专题	中国科学院西北高原生物研究所
通讯作者	Pei, Jinjin; Tao, Yanduo
作者单位	1.Shaanxi Univ Technol, Coll Biosci & Bioengn, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhong 723001, Shaanxi, Peoples R China 2.Chinese Acad Sci Xining, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810081, Qinghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Res, Xining 810081, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Pei, Jinjin,Jiang, Hai,Li, Xinsheng,et al. Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates[J]. AMB EXPRESS,2017,7.
APA	Pei, Jinjin,Jiang, Hai,Li, Xinsheng,Jin, Wengang,&Tao, Yanduo.(2017).Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates.AMB EXPRESS,7.
MLA	Pei, Jinjin,et al."Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates".AMB EXPRESS 7(2017).