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Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology
Cui, Yulei1,2,3,4; Shen, Na1,2,3,4; Wang, Shuo1,2,3; Mei, Lijuan1,2,3; Liu, Zenggen1,2,3; Dang, Jun1,2,3; Tao, Yanduo1,2,3
2017-11-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1068Pages:282-288
SubtypeArticle
AbstractTraditional Chinese medicine is important for discovery of drug precursors. However, information about trace chemical composition of them is very limited due to the lack of appropriate enrichment and chromatographic purification methods In our work, A. kansuensis was taken as an example, a novel two-dimensional reversed-phase/hydrophilic interaction liquid chromatography coupled with UniElut C18AEX solid-phase extraction re enrichment method based on anti-inflammatory bioactivity-guided assay was developed for gathering and purifying trace beta-carboline alkaloids with high purity from the ethyl acetate extract of A. kansuensis. Extraction with ethyl acetate as the first enrichment method, then, a UniElut C18AEX column was employed to re-enrich trace fraction which was hardly detected by diode array detector during high performance liquid chromatography analysis, eighteen grams of UniElut C18AFX was used as sorbent material to pack a 60 mL pipette tip for the extraction of beta-carboline alkaloids from 100 mL of ethyl acetate sample. The whole extraction process was finished in 10 min, and the volume of eluent used was only 120 mL. The enriching fraction (100 mg) was used for the following two-dimensional purification. First-dimensional preparation was carried on a RP-Megress-C18 prep column, and four anti-inflammatory fractions were obtained from the 100 mg re-enriching sample with a recovery of 66.9%. A HILIC-XAmide prep column was selected for the second dimensional preparation. Finally, two pair of analogue beta-carboline alkaloids and one other beta-carboline alkaloid were purified from A. kansuensis. The purity of the isolated compounds was >> > 98%, which indicated that the method was efficient to re-enrich and manufacture single trace beta-carboline alkaloids with high purity from A. kansuensis. Additionally, this method showed great potential to serve as a good example for the purification and enrichment of analogue structure anti-inflammation carboline alkaloids from other plant materials.
KeywordUnielut C18aex Spe Arenaria Kansuensis Two-dimensional Chromatography Trace Re-enrichment Beta-carboline Alkaloids
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2017.10.058
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PREPARATIVE ISOLATION ; CARBAMATE PESTICIDES ; RHEUMATOID-ARTHRITIS ; SAMPLE PRETREATMENT ; MICROEXTRACTION ; INFLAMMATION ; DISPERSION
Indexed BySCI
Language英语
Funding OrganizationWest Light Foundation of The Chinese Academy of Sciences for Doctors ; National Natural Science Foundation of China(31600262) ; Science and Technology Major Project of Qinghai Province, China(2014-GX-A3A) ; Strategic network planning project of biological resources service from Chinese academy of science(ZSTH-027) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A-01)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000417963100038
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13287
Collection中国科学院西北高原生物研究所
Corresponding AuthorDang, Jun; Tao, Yanduo
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China
2.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
3.Key Lab Tibetan Med Res Qinghai Prov, Xining 810008, Qinghai, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Cui, Yulei,Shen, Na,Wang, Shuo,et al. Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1068:282-288.
APA Cui, Yulei.,Shen, Na.,Wang, Shuo.,Mei, Lijuan.,Liu, Zenggen.,...&Tao, Yanduo.(2017).Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1068,282-288.
MLA Cui, Yulei,et al."Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1068(2017):282-288.
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