Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology

doi:10.1016/j.jchromb.2017.10.058

NWIPB OpenIR

	Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology
	Cui, Yulei 1,2,3,4; Shen, Na 1,2,3,4; Wang, Shuo 1,2,3; Mei, Lijuan 1,2,3; Liu, Zenggen1,2,3 ; Dang, Jun 1,2,3; Tao, Yanduo 1,2,3
	2017-11-15
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	1068 页码:282-288
文章类型	Article
摘要	Traditional Chinese medicine is important for discovery of drug precursors. However, information about trace chemical composition of them is very limited due to the lack of appropriate enrichment and chromatographic purification methods In our work, A. kansuensis was taken as an example, a novel two-dimensional reversed-phase/hydrophilic interaction liquid chromatography coupled with UniElut C18AEX solid-phase extraction re enrichment method based on anti-inflammatory bioactivity-guided assay was developed for gathering and purifying trace beta-carboline alkaloids with high purity from the ethyl acetate extract of A. kansuensis. Extraction with ethyl acetate as the first enrichment method, then, a UniElut C18AEX column was employed to re-enrich trace fraction which was hardly detected by diode array detector during high performance liquid chromatography analysis, eighteen grams of UniElut C18AFX was used as sorbent material to pack a 60 mL pipette tip for the extraction of beta-carboline alkaloids from 100 mL of ethyl acetate sample. The whole extraction process was finished in 10 min, and the volume of eluent used was only 120 mL. The enriching fraction (100 mg) was used for the following two-dimensional purification. First-dimensional preparation was carried on a RP-Megress-C18 prep column, and four anti-inflammatory fractions were obtained from the 100 mg re-enriching sample with a recovery of 66.9%. A HILIC-XAmide prep column was selected for the second dimensional preparation. Finally, two pair of analogue beta-carboline alkaloids and one other beta-carboline alkaloid were purified from A. kansuensis. The purity of the isolated compounds was >> > 98%, which indicated that the method was efficient to re-enrich and manufacture single trace beta-carboline alkaloids with high purity from A. kansuensis. Additionally, this method showed great potential to serve as a good example for the purification and enrichment of analogue structure anti-inflammation carboline alkaloids from other plant materials.
关键词	Unielut C18aex Spe Arenaria Kansuensis Two-dimensional Chromatography Trace Re-enrichment Beta-carboline Alkaloids
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.jchromb.2017.10.058
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PREPARATIVE ISOLATION ; CARBAMATE PESTICIDES ; RHEUMATOID-ARTHRITIS ; SAMPLE PRETREATMENT ; MICROEXTRACTION ; INFLAMMATION ; DISPERSION
收录类别	SCI
语种	英语
项目资助者	West Light Foundation of The Chinese Academy of Sciences for Doctors ; National Natural Science Foundation of China(31600262) ; Science and Technology Major Project of Qinghai Province, China(2014-GX-A3A) ; Strategic network planning project of biological resources service from Chinese academy of science(ZSTH-027) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A-01)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000417963100038
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13287
专题	中国科学院西北高原生物研究所
通讯作者	Dang, Jun; Tao, Yanduo
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China 2.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 3.Key Lab Tibetan Med Res Qinghai Prov, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cui, Yulei,Shen, Na,Wang, Shuo,et al. Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1068:282-288.
APA	Cui, Yulei.,Shen, Na.,Wang, Shuo.,Mei, Lijuan.,Liu, Zenggen.,...&Tao, Yanduo.(2017).Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1068,282-288.
MLA	Cui, Yulei,et al."Trace anti-inflammatory beta-carboline alkaloid identified in Arenaria kansuensis by two-dimensional chromatography coupled with UniElut C18AEX based solid-phase extraction re-enrichment technology".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1068(2017):282-288.