Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3

doi:10.1007/s13577-017-0188-4

NWIPB OpenIR

	Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3
	Yu, Rui-xue 1; Yu, Rui-tao 2,4; Liu, Zhong 3
	2018
发表期刊	HUMAN CELL
ISSN	1749-0774
卷号	31 期号:1 页码:50-63
文章类型	Article
摘要	Fucoxanthin is a natural carotenoid that had never been previously demonstrated to have anti-tumor effect on human gastric adenocarcinoma SGC-7901 or BGC-823 cells. Here it was found to inhibit proliferation and induce apoptosis through JAK/STAT signal pathway in these cells; the mechanism by which this occurred was investigated. We find that fucoxanthin significantly increased the number of apoptotic cells by propidium iodide (PI) dye staining and flow cytometry. Fucoxanthin (50 or 75 mu M) induced SGC-7901 cells cycle arrest at S phase, while BGC-823 cells arrest at G2/M phase. RT-PCR and western blot analysis revealed that the expressions of Mcl-1, STAT3 and p-STAT3 were obviously decreased by fucoxanthin in a dose-dependent manner. Synthetic siRNA targeting Mcl-1 was transfected into cells which had no effect on expressions of STAT3. After pretreatment with AG490 (50 mu M) which led to blocking of the JAK/STAT signal pathway, the reductive expressions of Mcl-1, STAT3 and p-STAT3 caused by fucoxanthin were inhibited. This is the first analysis of effects on SGC-7901 and BGC-823 cells by fucoxanthin. Fucoxanthin can induce cell-cycle arrest and apoptosis in these cells. These effects involved downregulation of Mcl-1, STAT3 and p-STAT3. This work is significant for better understanding of mechanisms leading to human gastric adenocarcinoma formation and informing exploitation of anti-tumor marine drug, and for providing Mcl-1 and STAT3 as potential therapeutic targets for gastric adenocarcinoma.
关键词	Fucoxanthin Gastric Adenocarcinoma Mcl-1 Stat3
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s13577-017-0188-4
关键词[WOS]	PATHWAY ; EXPRESSION ; APOPTOSIS ; SRC ; INFLAMMATION
收录类别	SCI
语种	英语
项目资助者	West Light Foundation of The Chinese Academy of Sciences ; National Natural Science Foundation of China(31101012) ; Qinghai Association for science and technology(2017KX05) ; International Cooperative Projects of Qinghai province(2017-HZ-811)
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000418841800007
出版者	SPRINGER JAPAN KK
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13288
专题	中国科学院西北高原生物研究所
通讯作者	Yu, Rui-tao; Liu, Zhong
作者单位	1.Pingdingshan Univ, Pingdingshan 467000, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 3.Chinese Acad Sci, Qinghai Inst Salt Lake, Xining 810008, Qinghai, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Yu, Rui-xue,Yu, Rui-tao,Liu, Zhong. Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3[J]. HUMAN CELL,2018,31(1):50-63.
APA	Yu, Rui-xue,Yu, Rui-tao,&Liu, Zhong.(2018).Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3.HUMAN CELL,31(1),50-63.
MLA	Yu, Rui-xue,et al."Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3".HUMAN CELL 31.1(2018):50-63.