NWIPB OpenIR
AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77
Cao, Dong1,3,4; Ye, Guangji2; Zong, Yuan2; Zhang, Bo1,4; Chen, Wenjie1,4; Liu, Baolong1,4; Zhang, Huaigang1,4
2017-12-01
发表期刊MOLECULES
ISSN1420-3049
卷号22期号:12
文章类型Article
摘要The red coleoptile trait can help monocotyledonous plants withstand stresses, and key genes responsible for the trait have been isolated from Triticum aestivum, Triticum urartu, and Triticum monococcum, but no corresponding research has been reported for Aegilops tauschii. In this research, transcriptome analysis was performed to isolate the candidate gene controlling the white coleoptile trait in Ae. tauschii. There were 5348 upregulated, differentially-expressed genes (DEGs) and 4761 downregulated DEGs in red coleoptile vs. white coleoptile plants. Among these DEGs, 12 structural genes and two transcription factors involved in anthocyanin biosynthesis were identified. The majority of structural genes showed lower transcript abundance in the white coleoptile of accession As77' than in the red coleoptile of accession As60', which implied that transcription factors related to anthocyanin biosynthesis could be the candidate genes. The MYB and MYC transcription factors AetMYB7D and AetMYC1 were both isolated from Ae. tauschii accessions As60' and As77', and their transcript levels analyzed. The coding sequence and transcript level of AetMYB7D showed no difference between As60' and As77'. AetMYC1p encoded a 567-amino acid polypeptide in As60' containing the entire characteristic domains, bHLH-MYC_N, HLH, and ACT-like, belonging to the gene family involved in regulating anthocyanin biosynthesis. AetMYC1w encoded a 436-amino acid polypeptide in As77' without the ACT-like domain because a single nucleotide mutation at 1310 bp caused premature termination. Transient expression of AetMYC1p induced anthocyanin biosynthesis in As77' with the co-expression of AetMYB7D, while AetMYC1w could not cause induced anthocyanin biosynthesis under the same circumstances. Moreover, the transcript abundance of AetMYC1w was lower than that of AetMYC1p. AetMYC1 appears to be the candidate gene controlling the white coleoptile trait in Ae. tauschii, which can be used for potential biotech applications, such as producing new synthetic hexaploid wheat lines with different coleoptile colors.
关键词Ae Tauschii Coleoptile Anthocyanin Biosynthesis Bhlh Transcription Factor Myb Transcription Factor
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.3390/molecules22122259
关键词[WOS]ANTHOCYANIN BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; WHEAT IMPROVEMENT ; TRITICUM-AESTIVUM ; REGULATORY GENES ; MAIZE ; EXPRESSION ; PIGMENTATION ; PERICARP ; RICE
收录类别SCI
语种英语
项目资助者Qinghai Science and Technology Department(2016-ZJ-Y01 ; Ministry of Science and Technology of China(2016YFD0100500) ; Chinese Academy of Sciences(XDA08030106) ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-06) ; 2017-ZJ-Y14)
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号WOS:000419242400221
出版者MDPI AG
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/13295
专题中国科学院西北高原生物研究所
通讯作者Liu, Baolong; Zhang, Huaigang
作者单位1.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China
2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 800010, Qinghai, Peoples R China
3.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China
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Cao, Dong,Ye, Guangji,Zong, Yuan,et al. AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77[J]. MOLECULES,2017,22(12).
APA Cao, Dong.,Ye, Guangji.,Zong, Yuan.,Zhang, Bo.,Chen, Wenjie.,...&Zhang, Huaigang.(2017).AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77.MOLECULES,22(12).
MLA Cao, Dong,et al."AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77".MOLECULES 22.12(2017).
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