Isolation, purification and identification of antioxidants from Lepidium latifolium extracts

doi:10.1007/s00044-017-2042-3

NWIPB OpenIR

	Isolation, purification and identification of antioxidants from Lepidium latifolium extracts
	Yuan Xiang 1,2; Wang Haixia 1; Mei Lijuan 1; Tao Yanduo 1
	2018
发表期刊	MEDICINAL CHEMISTRY RESEARCH
ISSN	1054-2523
卷号	27 期号:1 页码:37-45
文章类型	Article
摘要	Antioxidant potencies of an ethanolic extract and its components from Lepidium latifolium were investigated. In this study, we found that the ethyl acetate soluble fraction of L. latifolium was a rich source of antioxidant, resulting from its high total phenolic content. To determine the antioxidant components of the ethyl acetate fraction, a bioassay-guided fractionation approach using 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate), and ferric reducing antioxidant power assays were conducted. Nine compounds were isolated and their structures were identified by MS and NMR spectral data and comparison to reported data. They are Quercetin-3-O-beta-d-sophoroside-7-O-alpha-l-rhamnoside (1), Apetalumoside B6 (2), Kaempferol-3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (3), Kaempferol-7-O-alpha-l-rhamnopyranoside (4), Kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (5), Kaempferol-3-O-(2-O-feruloyl-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside)-7-O-glucopyranoside (6), Kaempferol-3-O-beta-D-sophoroside-7-O-alpha-l-rhamnoside (7), Kaempferol-3-O-robinoside-7-O-(2aEuro(3)aEuro(3)-(E)-feruloyl)-sophoroside (8), Quercetin-3-O-(2,6-di-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (9), compounds 1, 2, 4, and 8 had potent free radical scavenging activity. The IC50 values of these compounds were 9.8-12.3 and 7.4-31.4 mu g/mL in DPPH and ABTS assays, respectively. The results indicate that L. latifolium is a potential natural source of antioxidants to treat several diseases related to oxidant by-products of human metabolism.
关键词	Lepidium Latifolium Antioxidant Activity Bioassay-guided Column Chromatography Structures
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00044-017-2042-3
关键词[WOS]	GLYCOSIDES ; LEAVES ; FRUITS
收录类别	SCI
语种	英语
项目资助者	Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Program of Chinese Academy of Sciences(ZSTH-027)
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal
WOS记录号	WOS:000419972400004
出版者	SPRINGER BIRKHAUSER
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13299
专题	中国科学院西北高原生物研究所
通讯作者	Tao Yanduo
作者单位	1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100000, Peoples R China
推荐引用方式 GB/T 7714	Yuan Xiang,Wang Haixia,Mei Lijuan,et al. Isolation, purification and identification of antioxidants from Lepidium latifolium extracts[J]. MEDICINAL CHEMISTRY RESEARCH,2018,27(1):37-45.
APA	Yuan Xiang,Wang Haixia,Mei Lijuan,&Tao Yanduo.(2018).Isolation, purification and identification of antioxidants from Lepidium latifolium extracts.MEDICINAL CHEMISTRY RESEARCH,27(1),37-45.
MLA	Yuan Xiang,et al."Isolation, purification and identification of antioxidants from Lepidium latifolium extracts".MEDICINAL CHEMISTRY RESEARCH 27.1(2018):37-45.