Isolation, purification and identification of antioxidants from Lepidium latifolium extracts
Yuan Xiang1,2; Wang Haixia1; Mei Lijuan1; Tao Yanduo1
AbstractAntioxidant potencies of an ethanolic extract and its components from Lepidium latifolium were investigated. In this study, we found that the ethyl acetate soluble fraction of L. latifolium was a rich source of antioxidant, resulting from its high total phenolic content. To determine the antioxidant components of the ethyl acetate fraction, a bioassay-guided fractionation approach using 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate), and ferric reducing antioxidant power assays were conducted. Nine compounds were isolated and their structures were identified by MS and NMR spectral data and comparison to reported data. They are Quercetin-3-O-beta-d-sophoroside-7-O-alpha-l-rhamnoside (1), Apetalumoside B6 (2), Kaempferol-3-O-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (3), Kaempferol-7-O-alpha-l-rhamnopyranoside (4), Kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (5), Kaempferol-3-O-(2-O-feruloyl-beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranoside)-7-O-glucopyranoside (6), Kaempferol-3-O-beta-D-sophoroside-7-O-alpha-l-rhamnoside (7), Kaempferol-3-O-robinoside-7-O-(2aEuro(3)aEuro(3)-(E)-feruloyl)-sophoroside (8), Quercetin-3-O-(2,6-di-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside-7-O-alpha-l-rhamnopyranoside (9), compounds 1, 2, 4, and 8 had potent free radical scavenging activity. The IC50 values of these compounds were 9.8-12.3 and 7.4-31.4 mu g/mL in DPPH and ABTS assays, respectively. The results indicate that L. latifolium is a potential natural source of antioxidants to treat several diseases related to oxidant by-products of human metabolism.
KeywordLepidium Latifolium Antioxidant Activity Bioassay-guided Column Chromatography Structures
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationProject of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Program of Chinese Academy of Sciences(ZSTH-027)
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000419972400004
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorTao Yanduo
Affiliation1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100000, Peoples R China
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GB/T 7714
Yuan Xiang,Wang Haixia,Mei Lijuan,et al. Isolation, purification and identification of antioxidants from Lepidium latifolium extracts[J]. MEDICINAL CHEMISTRY RESEARCH,2018,27(1):37-45.
APA Yuan Xiang,Wang Haixia,Mei Lijuan,&Tao Yanduo.(2018).Isolation, purification and identification of antioxidants from Lepidium latifolium extracts.MEDICINAL CHEMISTRY RESEARCH,27(1),37-45.
MLA Yuan Xiang,et al."Isolation, purification and identification of antioxidants from Lepidium latifolium extracts".MEDICINAL CHEMISTRY RESEARCH 27.1(2018):37-45.
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