DYNAMIC DISTRIBUTION OF CALCIUM IN THE STIGMA AND STYLE OF LILY AFTER POLLINATION

NWIPB OpenIR

	DYNAMIC DISTRIBUTION OF CALCIUM IN THE STIGMA AND STYLE OF LILY AFTER POLLINATION
	Tang, Nan 1,2; Yan, Jinyan 3; Li, Yi2 ; Tang, Daocheng 1,3
	2017-12-01
发表期刊	PAKISTAN JOURNAL OF BOTANY
ISSN	0556-3321
卷号	49 期号:6 页码:2419-2427
文章类型	Article
摘要	Lilies are ornamental plants of global importance, yet cross incompatibility remains a major problem in breeding programmes. Calcium plays an essential role in the sexual reproduction of flowering plants. However, the regulatory mechanisms of calcium distribution during the fertilization process are unclear at present. An accurate assessment of calcium ions in pollinated styles can help to substantiate the role of calcium in pollination. In the current study, Lilium pumilum DC. and two Asiatic Lilium cultivars ('Pollyanna' and 'Prato') were used for crossing. Three cross combinations with different compatibilities were selected. The style of the female parent was collected before and after pollination at different stages. The amount of exudate in the stylar canal was estimated at the same time points. The style was cut into four different segments using an ultramicrotome and calcium ions were localized using potassium pyroantimonate. Calcium precipitates were examined and photographed using transmission electron microscopy. The results showed that the amount of exudate in the style increased prominently following pollination in compatible crosses ('Prato' x 'Pollyanna'). The amount of calcium in the stigma increased after pollination, particularly significantly so in incompatible crosses ('Prato' x L. pumilum). The amount of calcium in the canal cells and vascular bundles of the style was greater than in the adjacent parenchyma cells. In the style of compatible crosses, calcium was obviously transferred from the top to the base in the canal cells and vascular bundles, and a rising calcium gradient was ultimately formed. It is speculated that a rapid increase and high concentration of calcium in the stigma of the female parent could negatively impact pollen tube growth. Instead, abundant calcium in the style appears to promote the growth of the pollen tube towards the ovary.
关键词	Lilium Pumilum Calcium Potassium Pyroantimonate Stigma Style Lily
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SELF-INCOMPATIBILITY RESPONSE ; GROWING POLLEN TUBES ; RUSCUS-ACULEATUS L ; LILIUM-LONGIFLORUM ; PAPAVER-RHOEAS ; CANAL EXUDATE ; IN-VITRO ; GROWTH ; GERMINATION ; IONS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31660582) ; amp ; apos ; State Key Laboratory Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilizationamp ; apos ; (2013-07)
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000419150400043
出版者	PAKISTAN BOTANICAL SOC
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13300
专题	中国科学院西北高原生物研究所
通讯作者	Li, Yi; Tang, Daocheng
作者单位	1.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 3.Qinghai Univ, Plateau Flower Res Ctr, Key Lab Qinghai Prov Landscape Plants Res, Xining 810016, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Tang, Nan,Yan, Jinyan,Li, Yi,et al. DYNAMIC DISTRIBUTION OF CALCIUM IN THE STIGMA AND STYLE OF LILY AFTER POLLINATION[J]. PAKISTAN JOURNAL OF BOTANY,2017,49(6):2419-2427.
APA	Tang, Nan,Yan, Jinyan,Li, Yi,&Tang, Daocheng.(2017).DYNAMIC DISTRIBUTION OF CALCIUM IN THE STIGMA AND STYLE OF LILY AFTER POLLINATION.PAKISTAN JOURNAL OF BOTANY,49(6),2419-2427.
MLA	Tang, Nan,et al."DYNAMIC DISTRIBUTION OF CALCIUM IN THE STIGMA AND STYLE OF LILY AFTER POLLINATION".PAKISTAN JOURNAL OF BOTANY 49.6(2017):2419-2427.