PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis

doi:10.1093/treephys/tpx093

NWIPB OpenIR

	PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis
	Xu, Changzheng 1; Fu, Xiaokang 1; Liu, Rui 1; Guo, Li 1; Ran, Lingyu 1; Li, Chaofeng 2; Tian, Qiaoyan 1; Jiao, Bo 1; Wang, Bangjun 1; Luo, Keming 1,2
	2017-12-01
发表期刊	TREE PHYSIOLOGY
ISSN	0829-318X
卷号	37 期号:12 页码:1713-1726
文章类型	Article
摘要	Wood formation is a complex developmental process under multi-level transcriptional control executed by a large set of transcription factors. However, only limited members have been characterized to be key regulators of lignin biosynthesis in poplar. Here we report the conserved and unique functions of PtoMYB170, a transcription factor identified from Populus tomentosa (Chinese white poplar), in lignin deposition and drought tolerance in comparison with its duplicate paralog PtoMYB216. PtoMYB170 is preferentially expressed in young leaves and xylem tissues. Overexpression of PtoMYB170 in transgenic poplar plants resulted in stronger lignification and more thickened secondary wall in xylem compared with wild-type plants, whereas the CRISPR/Cas9-generated mutation of PtoMYB170 weakened lignin deposition, thereby leading to a more flexible and collapsed xylem phenotype. Transient expression experiments demonstrated that PtoMYB170 specifically activated the expression of lignin biosynthetic genes, consistent with the function of PtoMYB216. However, GUS staining assays revealed that PtoMYB170 was specifically expressed in guard cells of transgenic Arabidopsis while PtoMYB216 was not. Heterologous expression of PtoMYB170 in Arabidopsis enhanced stomatal closure in the dark and resulted in drought tolerance of the transgenic plants through reduced water loss, indicating a diversified role from PtoMYB216. These results revealed the PtoMYB170-dependent positive transcriptional regulation on lignin deposition in poplar and its coordinated function in enhancing drought tolerance by promoting dark-induced stomatal closure.
关键词	Drought Tolerance Myb Transcription Factor Populus Tomentosa Stomatal Aperture Wood Formation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1093/treephys/tpx093
关键词[WOS]	SECONDARY WALL BIOSYNTHESIS ; MYB TRANSCRIPTION FACTORS ; DIRECT TARGET ; R2R3-MYB ; POPULUS ; GENOME ; PLANTS ; LIGNIFICATION ; THALIANA ; ATMYB61
收录类别	SCI
语种	英语
项目资助者	National Key Research and Development Program(2016YFD0600105) ; National Natural Science Foundation of China(31370672 ; Chongqing Research Program of Basic Research and Frontier Technology(cstc2015jcyjA80036 ; Fundamental Research Funds for the Central Universities(XDJK2016B032) ; 31500216 ; cstc2013jcyjA80016) ; 31500544 ; 31370317)
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000422839200011
出版者	OXFORD UNIV PRESS
引用统计	被引频次：79[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13301
专题	中国科学院西北高原生物研究所
通讯作者	Luo, Keming
作者单位	1.Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Xu, Changzheng,Fu, Xiaokang,Liu, Rui,et al. PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis[J]. TREE PHYSIOLOGY,2017,37(12):1713-1726.
APA	Xu, Changzheng.,Fu, Xiaokang.,Liu, Rui.,Guo, Li.,Ran, Lingyu.,...&Luo, Keming.(2017).PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis.TREE PHYSIOLOGY,37(12),1713-1726.
MLA	Xu, Changzheng,et al."PtoMYB170 positively regulates lignin deposition during wood formation in poplar and confers drought tolerance in transgenic Arabidopsis".TREE PHYSIOLOGY 37.12(2017):1713-1726.