Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline

doi:10.1016/j.chroma.2017.11.053

NWIPB OpenIR

	Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline
	Zheng, Longfang 1,2; Zhao, Xian-En 1,2; Ji, Wenhua 3; Wang, Xiao 3; Tao, Yanduo 4; Sun, Jing 4; Xu, Yanqiu 1,2; Wang, Xin 1,2; Zhu, Shuyun 1,2; You, Jinmao1,2,4
	2018-01-12
发表期刊	JOURNAL OF CHROMATOGRAPHY A
ISSN	0021-9673
卷号	1532 页码:30-39
文章类型	Article
摘要	In this work, a novel core-shell magnetic molecularly imprinted polymers (MMIPs) for the measurement of L-Hydroxyproline (Hyp) in dairy products was prepared. The derivative of Hyp using N-hydroxysuccinimidyl rhodamine B ester (RBS) as derivatization reagent was employed as template to prepare RBS-Hyp-MMIPs (Fe3O4@MIPs for RBS-Hyp). A new analytical procedure of in situ derivatization with MMIPs (ISD-MMIPs) has been developed for the specific extraction and determination of Hyp in dairy products by ultra high performance liquid chromatography tandem mass spectrometry. The RBS-Hyp-MMIPs was characterized by fourier transform infrared spectrometer and transmission electron microscopy, and evaluated by adsorption experiments. The adsorption process followed Langumuir adsorption isotherm with maximum adsorption capacity of RBS-Hyp on RBS-Hyp-MMIPs at 96 mg/g. In addition, RBS-Hyp-MMIPs showed a short equilibrium time (15.0 min), rapid magnetic separation (5 s) and high stability (retained 95.3% after six cycles). Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N >10) at 0.1 and 0.5 ng/mL, respectively. On account of the specific extraction performance of RBS-Hyp-MMIPs, not any interference peak from real sample matrix was observed in the chromatograms of milk powder, liquid milk and milk drink. The proposed procedure was successfully applied for selective determination of Hyp from dairy products with satisfactory validation results, which is of great significance to food safety. (C) 2017 Elsevier B.V. All rights reserved.
关键词	L-hydroxyproline In Situ Derivatization Magnetic Molecularly Imprinted Polymer Specific Extraction Dairy Products
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.chroma.2017.11.053
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; LIQUID-LIQUID MICROEXTRACTION ; ENVIRONMENTAL WATER SAMPLES ; ASSISTED DERIVATIZATION ; FLUORESCENCE DETECTION ; BORONATE AFFINITY ; CARBON NANOTUBES ; LABELING REAGENT ; DAIRY-PRODUCTS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21775088 ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Open Projects Program of the Key Laboratory of Tibetan Medicine Research of Chinese Academy of Sciences ; Open Funds of Key Laboratory of TCM Quality Control Technology of Shandong Academy of Sciences ; Experimental Research Project of Qufu Normal University(sj201402) ; Foundation of Qufu Normal University(BSQD2012019 ; 81603286 ; BSQD2012023) ; 21405094 ; 81303179)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000423894500004
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13306
专题	中国科学院西北高原生物研究所
通讯作者	Zhao, Xian-En; Ji, Wenhua; You, Jinmao
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Longfang,Zhao, Xian-En,Ji, Wenhua,et al. Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1532:30-39.
APA	Zheng, Longfang.,Zhao, Xian-En.,Ji, Wenhua.,Wang, Xiao.,Tao, Yanduo.,...&You, Jinmao.(2018).Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline.JOURNAL OF CHROMATOGRAPHY A,1532,30-39.
MLA	Zheng, Longfang,et al."Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline".JOURNAL OF CHROMATOGRAPHY A 1532(2018):30-39.