NWIPB OpenIR
Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline
Zheng, Longfang1,2; Zhao, Xian-En1,2; Ji, Wenhua3; Wang, Xiao3; Tao, Yanduo4; Sun, Jing4; Xu, Yanqiu1,2; Wang, Xin1,2; Zhu, Shuyun1,2; You, Jinmao1,2,4
2018-01-12
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1532Pages:30-39
SubtypeArticle
AbstractIn this work, a novel core-shell magnetic molecularly imprinted polymers (MMIPs) for the measurement of L-Hydroxyproline (Hyp) in dairy products was prepared. The derivative of Hyp using N-hydroxysuccinimidyl rhodamine B ester (RBS) as derivatization reagent was employed as template to prepare RBS-Hyp-MMIPs (Fe3O4@MIPs for RBS-Hyp). A new analytical procedure of in situ derivatization with MMIPs (ISD-MMIPs) has been developed for the specific extraction and determination of Hyp in dairy products by ultra high performance liquid chromatography tandem mass spectrometry. The RBS-Hyp-MMIPs was characterized by fourier transform infrared spectrometer and transmission electron microscopy, and evaluated by adsorption experiments. The adsorption process followed Langumuir adsorption isotherm with maximum adsorption capacity of RBS-Hyp on RBS-Hyp-MMIPs at 96 mg/g. In addition, RBS-Hyp-MMIPs showed a short equilibrium time (15.0 min), rapid magnetic separation (5 s) and high stability (retained 95.3% after six cycles). Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N >10) at 0.1 and 0.5 ng/mL, respectively. On account of the specific extraction performance of RBS-Hyp-MMIPs, not any interference peak from real sample matrix was observed in the chromatograms of milk powder, liquid milk and milk drink. The proposed procedure was successfully applied for selective determination of Hyp from dairy products with satisfactory validation results, which is of great significance to food safety. (C) 2017 Elsevier B.V. All rights reserved.
KeywordL-hydroxyproline In Situ Derivatization Magnetic Molecularly Imprinted Polymer Specific Extraction Dairy Products
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.11.053
WOS KeywordTANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; LIQUID-LIQUID MICROEXTRACTION ; ENVIRONMENTAL WATER SAMPLES ; ASSISTED DERIVATIZATION ; FLUORESCENCE DETECTION ; BORONATE AFFINITY ; CARBON NANOTUBES ; LABELING REAGENT ; DAIRY-PRODUCTS
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21775088 ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Open Projects Program of the Key Laboratory of Tibetan Medicine Research of Chinese Academy of Sciences ; Open Funds of Key Laboratory of TCM Quality Control Technology of Shandong Academy of Sciences ; Experimental Research Project of Qufu Normal University(sj201402) ; Foundation of Qufu Normal University(BSQD2012019 ; 81603286 ; BSQD2012023) ; 21405094 ; 81303179)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000423894500004
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13306
Collection中国科学院西北高原生物研究所
Corresponding AuthorZhao, Xian-En; Ji, Wenhua; You, Jinmao
Affiliation1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Longfang,Zhao, Xian-En,Ji, Wenhua,et al. Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1532:30-39.
APA Zheng, Longfang.,Zhao, Xian-En.,Ji, Wenhua.,Wang, Xiao.,Tao, Yanduo.,...&You, Jinmao.(2018).Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline.JOURNAL OF CHROMATOGRAPHY A,1532,30-39.
MLA Zheng, Longfang,et al."Core-shell magnetic molecularly imprinted polymers used rhodamine B hydroxyproline derivate as template combined with in situ derivatization for the specific measurement of L-hydroxyproline".JOURNAL OF CHROMATOGRAPHY A 1532(2018):30-39.
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