The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep

doi:10.1016/j.theriogenology.2017.10.039

NWIPB OpenIR

	The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep
	Zhang, Rui-Na 1; Pang, Bo 1; Xu, Shang-Rong 2; Wan, Peng-Cheng 3; Guo, Song-Chang 1; Ji, Han-Zhong 4; Jia, Gong-Xue 1; Hu, Lin-Yong1 ; Zhao, Xin-Quan1 ; Yang, Qi-En 1,5
	2018-02-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	107 页码:85-94
文章类型	Article
摘要	Gonadotropins and growth factors synergistically regulate folliculogenesis and oocyte development. C-XC motif chemokine 12 (CXCL12) and its receptor CXCR4 are expressed in ovaries of sheep, cattle and other species, however, roles of this multifunctional signal axis in oocyte maturation are not defined. Using sheep as a model, we examined the expression patterns and functions of the CXCL12-CXCR4 axis during oocyte maturation. CXCL12 and CXCR4 mRNA and protein were present in oocytes and granulosa cells. Relative abundance of CXCR4 transcript was controlled by epidermal growth factor (EGF). Transient inhibition of CXCR4 suppressed oocyte nuclear maturation while supplementing recombination CXCL12 significantly increased percent of oocyte undergone metaphase I phase. Inhibition of CXCR4 function decreased cumulus expansion growth rate. Furthermore, granulosa cell migration was impaired and expression of hyaluronan synthase 2 (HAS2) and hyaluronan binding protein tumor necrosis factor alpha-induced protein 6 (TNFAIP6) were downregulated by CXCR4 inhibition. These findings revealed a novel role of the CXCL12-CXCR4 signaling in oocyte development in sheep. (C) 2017 Elsevier Inc. All rights reserved.
关键词	Sheep Ovary Cxcl12 Cxcr4 Oocyte In Vitro Maturation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.theriogenology.2017.10.039
关键词[WOS]	GROWTH-FACTOR RECEPTOR ; FOLLICLE-STIMULATING-HORMONE ; HYALURONIC-ACID SYNTHESIS ; FACTOR-I ; DEVELOPMENTAL COMPETENCE ; PREOVULATORY FOLLICLES ; OVARIAN-FOLLICLE ; CYTOPLASMIC MATURATION ; CXCR4 ; MOUSE
收录类别	SCI
语种	英语
项目资助者	National Key Research & Development Project(2016YFC0501805) ; Qinghai Department of Science and Technology(2015-ZJ-714 ; CAS "100 Talents" program ; Qinghai "1000 Talents" program ; CPSF-CAS ; CAS "Light of West China Foundation" ; 2016-ZJ-917Q)
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:000422892800012
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13311
专题	中国科学院西北高原生物研究所
通讯作者	Zhao, Xin-Quan; Yang, Qi-En
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Evolut & Adaptat Plateau Biota, Xining 810008, Qinghai, Peoples R China 2.Qinghai Univ, Qinghai Acad Anim Sci & Vet Med, Xining 810016, Qinghai, Peoples R China 3.Xinjiang Acad Agr & Reclamat Sci, Anim Husb & Vet Inst, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832003, Peoples R China 4.Haibei Inst Anim Husb & Vet Sci, Haibei 810299, Tibetan Autonom, Peoples R China 5.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Ecol Genom, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rui-Na,Pang, Bo,Xu, Shang-Rong,et al. The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep[J]. THERIOGENOLOGY,2018,107:85-94.
APA	Zhang, Rui-Na.,Pang, Bo.,Xu, Shang-Rong.,Wan, Peng-Cheng.,Guo, Song-Chang.,...&Yang, Qi-En.(2018).The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep.THERIOGENOLOGY,107,85-94.
MLA	Zhang, Rui-Na,et al."The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep".THERIOGENOLOGY 107(2018):85-94.