Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East

doi:10.1007/s10722-017-0560-y

NWIPB OpenIR

	Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East
	Cao, Dong 1,3,4,5; Ye, Guangji 2,5; Zong, Yuan 2,5; Zhang, Bo 1,3,5; Chen, Wenjie 1,3,5; Liu, Baolong 1,3,5; Zhang, Huaigang1,3,5
	2018-02-01
发表期刊	GENETIC RESOURCES AND CROP EVOLUTION
ISSN	0925-9864
卷号	65 期号:2 页码:637-644
文章类型	Article
摘要	Rc1 (red coleoptile) encode a R2R3-MYB transcription factor TaMYB-A1 in common wheat, but the homologous genes have not been reported in Triticum urartu or Triticum monococcum, both species being sources of the ancient A genome. In this study, four alleles were discovered in 120 T. urartu accessions, and two alleles from nine T. monococcum accessions. The six alleles encoded five proteins because TuMYB-A1-F and TuMYB-A1-C encoded the same protein. All mutated sites in the amino acid sequence of these alleles were out of the R2R3 and transcript activator domains, and did not affect the conserved tryptophan (W) residues nor the "D/ELX2R/KX3LX6LX3R" motif of the MYB proteins. The alleles from T. monococcum were more similar in sequence to TaMYB-B1 and TaMYB-D1 than were those from T. urartu in the phylogenetic tree, while the alleles from T. urartu resembled TaMYB-A1 more closely than did the T. monococcum alleles. TuMYB-A1-C was the most widely distributed allele, and Turkey contained the greatest genetic variation compared with the five other countries in the geographical distribution analysis. Transient expression of TuMYB-A1-D or TuMYB-A1-F induced anthocyanin biosynthesis in white coleoptiles of wheat cultivar 'Opata' with the help of ZmR. RT-PCR analysis showed that the transcript level of Rc1 allele in white coleoptiles was clearly lower than in red coleoptiles, suggesting that Rc1 allele plays an important role in anthocyanin biosynthesis in coleoptiles of T. urartu. The allele isolation, phylogenetic analysis, geographical distribution and functional verification should be helpful in understanding the functions of Rc1 alleles and the molecular mechanism underlying the coleoptile color trait in Triticum.
关键词	Triticum Urartu Rc1 Red Coleoptile Myb Transcription Factor Geographical Distribution
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s10722-017-0560-y
关键词[WOS]	ANTHOCYANIN ACCUMULATION ; BIOSYNTHESIS GENES ; WHEAT ; COLEOPTILE ; STRESS ; COLOR ; DOMESTICATION ; PIGMENTATION ; TEMPERATURE ; EXPRESSION
收录类别	SCI
语种	英语
项目资助者	Project of Qinghai Science and Technology Department(2016-ZJ-Y01 ; West Light Foundation of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030106) ; Ministry of Science and Technology of China(2016YFD0100500) ; Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-06) ; 2017-ZJ-Y14)
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agronomy ; Plant Sciences
WOS记录号	WOS:000424022000021
出版者	SPRINGER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13313
专题	中国科学院西北高原生物研究所
通讯作者	Chen, Wenjie; Liu, Baolong
作者单位	1.Key Lab Crop Mol Breeding Qinghai Prov, Xining 810008, Qinghai, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 800010, Qinghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Key Lab Qinghai Prov Plateau Crop Germplasm Innov, State Key Lab Breeding Base, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Cao, Dong,Ye, Guangji,Zong, Yuan,et al. Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East[J]. GENETIC RESOURCES AND CROP EVOLUTION,2018,65(2):637-644.
APA	Cao, Dong.,Ye, Guangji.,Zong, Yuan.,Zhang, Bo.,Chen, Wenjie.,...&Zhang, Huaigang.(2018).Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East.GENETIC RESOURCES AND CROP EVOLUTION,65(2),637-644.
MLA	Cao, Dong,et al."Diversity and distribution of Rc1 alleles in Triticum urartu from the Middle East".GENETIC RESOURCES AND CROP EVOLUTION 65.2(2018):637-644.