Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles | |
Qu, Fei1,2; Huang, Wei1,2; You, Jinmao1,2,3 | |
2018-02-01 | |
发表期刊 | COLLOIDS AND SURFACES B-BIOINTERFACES |
卷号 | 162页码:212-219 |
文章类型 | Article |
摘要 | In this work, we report a fluorescence strategy for detecting dopamine (DA) and sensing tyrosinase (TYR) activity on the basis of the dual-emission carbon dots (DECDs), which contain two emitters: the blue emitters (BE, maximum emission at 385 nm) and yellow emitters (YE, maximum emission at 530 nm). Gold nanoparticles (AuNPs) can effectively quench the two emissions of DECDs. The addition of DA aggregates AuNPs effectively, leading to the fluorescence recovery of dual emitters gradually. This strategy exhibits a high selectivity toward DA and shows good linear ranges, such as 0.5-3 mu M for BE and 0.1-3 mu M for YE. Additionally, the proposed method is successfully applied to the determination of DA in real samples with satisfactory recoveries. Subsequently, this DECDs-AuNPs platform is further taken advantage to assess TYR activity by the aid of TYR's capability for oxidation of DA into dopaquinone, which will not induce the agglomeration of AuNPs, so the fluorescence quenching of DECDs is associated with TYR activity. Finally, the mechanism of the reaction is discussed in detail, and the results suggest that both amine and phenolic hydroxyl groups of DA bring the aggregation of AuNPs. (C) 2017 Elsevier B.V. All rights reserved. |
关键词 | Dual-emission Carbon Dots Gold Nanoparticles Dopamine Tyrosinase Activity |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
DOI | 10.1016/j.colsurfb.2017.11.055 |
关键词[WOS] | GRAPHENE QUANTUM DOTS ; NANODOTS ; PROBE ; NANOCLUSTERS ; STRATEGY ; CELLS ; WATER ; ASSAY |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(21405093) ; Scientific Research Foundation of Qufu Normal University(BSQD20130117) |
WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
WOS类目 | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
WOS记录号 | WOS:000425076500025 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/13331 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Qu, Fei,Huang, Wei,You, Jinmao. A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018,162:212-219. |
APA | Qu, Fei,Huang, Wei,&You, Jinmao.(2018).A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles.COLLOIDS AND SURFACES B-BIOINTERFACES,162,212-219. |
MLA | Qu, Fei,et al."A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles".COLLOIDS AND SURFACES B-BIOINTERFACES 162(2018):212-219. |
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