Simple and label-free fluorescence detection of ascorbic acid in rat brain microdialysates in the presence of catecholamines

doi:10.1039/c7nj04574c

NWIPB OpenIR

	Simple and label-free fluorescence detection of ascorbic acid in rat brain microdialysates in the presence of catecholamines
	Zhu, Shuyun 1,2; Lei, Cuihua 1; Gao, Yue 1; Sun, Jing 2; Peng, Hongwei 3; Gao, Han 1; Zhang, Ruixue 1; Wang, Rui 1; Zhao, Xian-En 1,2; Wang, Hua 1
	2018-03-07
发表期刊	NEW JOURNAL OF CHEMISTRY
卷号	42 期号:5 页码:3851-3856
文章类型	Article
摘要	Ascorbic acid (AA), as one of the most important neurochemicals in cerebral systems, plays a vital role in many physiological and pathological processes. Herein, a facile, label-free, and ultrasensitive fluorescence sensing system was developed for the determination of AA based on the Ag+-o-phenylenediamine (OPD) interaction. OPD could be oxidized by Ag+ to generate fluorescent 2,3-diaminophenazine (OPDox). When AA was introduced, on the one hand, AA can inhibit the oxidation process due to its strong reducing capability. On the other hand, AA can be oxidized to dehydro-AA with an absorption peak at 380 nm, which has a good spectral overlap with the emission of OPDox. Thus, the inner filter effect (IFE) between dehydro-AA and OPDox may occur. Therefore, the introduction of AA can intensively suppress the fluorescence of the Ag+-OPD system. Benefitting from the remarkable synergistic effect of the reducing capability of AA and IFE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The whole detection procedure was achieved within 10 min. The linear response range of AA was obtained from 0.05 to 40 M with a detection limit of 10 nM. This developed method has many merits including more simplicity, good selectivity, excellent biocompatibility, and more cost-effectiveness without using any nanomaterials. Notably, the proposed method was successfully applied to detect AA in rat brain microdialysates in the presence of catecholamines, thus providing a new route for AA detection in physiological and pathological fields.
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1039/c7nj04574c
关键词[WOS]	QUANTUM DOTS ; IN-VIVO ; O-PHENYLENEDIAMINE ; IONS ; ISCHEMIA ; SENSOR ; NANOCLUSTERS ; ANTIOXIDANT ; NANOPROBE ; OXIDATION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21405094 ; Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Natural Science Foundation of Qinghai Province(2016-ZJ-955) ; Development Project of Qinghai Key Laboratory(2017-ZJ-Y10) ; National Undergraduate Research Training Program(201610446033 ; Scientific Research Foundation of Qufu Normal University(BSQD201203) ; 21775088 ; 2017A029) ; 21675099)
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000426488900078
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13340
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Inst Med & Mat Appl Technol, Qufu City 273165, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 3.Qufu Normal Univ, Univ Hosp, Qufu City 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Shuyun,Lei, Cuihua,Gao, Yue,et al. Simple and label-free fluorescence detection of ascorbic acid in rat brain microdialysates in the presence of catecholamines[J]. NEW JOURNAL OF CHEMISTRY,2018,42(5):3851-3856.
APA	Zhu, Shuyun.,Lei, Cuihua.,Gao, Yue.,Sun, Jing.,Peng, Hongwei.,...&Wang, Hua.(2018).Simple and label-free fluorescence detection of ascorbic acid in rat brain microdialysates in the presence of catecholamines.NEW JOURNAL OF CHEMISTRY,42(5),3851-3856.
MLA	Zhu, Shuyun,et al."Simple and label-free fluorescence detection of ascorbic acid in rat brain microdialysates in the presence of catecholamines".NEW JOURNAL OF CHEMISTRY 42.5(2018):3851-3856.