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Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS
Wang, HL; Li, YL; Ding, CX; Zhao, XN; You, JM; Suo, YR
2006
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume29Issue:6Pages:857-868
SubtypeArticle
AbstractA rapid and sensitive liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) assay for the determination of five pharmacologically active compounds (PAC) extracted from the traditional Chinese medicine, Rhodiola , namely salidroside, tyrosol, rhodionin, gallic acid, and ethyl gallate has been developed. In this method, PAC could be baseline separated and detected with DAD at 275 nm. The validation of the method, including sensitivity, linearity, repeatability, and recovery, was examined. The linear calibration curves were acquired with correlation coefficient >0.999 and the limits of detection LOD (at a signal-to-noise ratio=3:1) were between 0.058 and 1.500 mu mol/L. It was found, that the amounts of PAC varied with different species of Rhodiola . The established method is rapid and reproducible for the separation of five natural pharmacologically active compounds from extracts of Rhodiola with satisfactory results.; A rapid and sensitive liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) assay for the determination of five pharmacologically active compounds (PAC) extracted from the traditional Chinese medicine, Rhodiola , namely salidroside, tyrosol, rhodionin, gallic acid, and ethyl gallate has been developed. In this method, PAC could be baseline separated and detected with DAD at 275 nm. The validation of the method, including sensitivity, linearity, repeatability, and recovery, was examined. The linear calibration curves were acquired with correlation coefficient >0.999 and the limits of detection LOD (at a signal-to-noise ratio=3:1) were between 0.058 and 1.500 mu mol/L. It was found, that the amounts of PAC varied with different species of Rhodiola . The established method is rapid and reproducible for the separation of five natural pharmacologically active compounds from extracts of Rhodiola with satisfactory results.
KeywordHigh Performance Liquid Chromatography Atmospheric Pressure Chemical Ionization Mass Spectrometry Pharmacologically Active Compounds Rhodiola
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Subject Area生物化学
WOS KeywordMICELLAR ELECTROKINETIC CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; POLYPHENOLS ; SEPARATION ; ACID
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000235552900007
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1343
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Qufu Normal Univ, Dept Chem, Qufu, Peoples R China
Recommended Citation
GB/T 7714
Wang, HL,Li, YL,Ding, CX,et al. Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(6):857-868.
APA Wang, HL,Li, YL,Ding, CX,Zhao, XN,You, JM,&Suo, YR.(2006).Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(6),857-868.
MLA Wang, HL,et al."Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.6(2006):857-868.
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