Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission

doi:10.1007/s00604-018-2939-7

NWIPB OpenIR

	Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission
	Yan, Xiaolu 1; Sun, Jing 2; Zhao, Xian-En 1; Wang, Renjun 3; Wang, Xiao 4; Zuo, Ya-Nan 1; Liu, Wei4 ; Kong, Rongmei 1; Zhu, Shuyun 1
	2018-09-01
发表期刊	MICROCHIMICA ACTA
ISSN	0026-3672
卷号	185 期号:9
文章类型	Article
摘要	The authors describe a molecular beacon-based fluorescent probe for the determination of the cancer drug bleomycin (BLM). The probe was tagged with DNA-templated silver nanoclusters (DNA-AgNCs) and guanine-rich sequences (GRSs) at two terminals serving as signal reporter with a loop. In the absence of the BLM-iron(II) complex [BLM-Fe(II)], the probe has a hairpin shape and displays strong fluorescence because the AgNCs are close to the GRSs. In the presence of the BLM-Fe(II) complex, it will selectively cleave the probe at the 5'-GC-3' scission site of the loop. This displaces the AgNCs away from the GRSs and causes a decrease in fluorescence, best measured at excitation/emission wavelengths of 565/623 nm. This effect enables BLM to be detected with a detection limit as low as 33 pM, which was 1-3 orders of magnitude more sensitive than most of the previous reports. The probe was applied for the determination of BLM in spiked human serum samples, and excellent performance was achieved. In our perception, the method described here represents a promising tool for highly sensitive and specific analysis of BLM during cancer treatment.
关键词	Fluorescence Dna-templated Synthesis Silver Nanoclusters Bleomycin Guanine-rich Sequence Antitumor Drug Dna Scission
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1007/s00604-018-2939-7
关键词[WOS]	GLUTATHIONE-REDUCTASE ACTIVITY ; GRAPHENE QUANTUM DOTS ; TURN-ON DETECTION ; LABEL-FREE ; COPPER NANOCLUSTERS ; GOLD NANOPARTICLES ; SENSING PLATFORM ; QUANTIFICATION ; IDENTIFICATION ; AMPLIFICATION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2017MC037 ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Development Project of Qinghai Key Laboratory(2017-ZJ-Y10) ; Open Funds of Shandong Key Laboratory of TCM Quality Control Technology of Shandong Academy of Sciences ; 21775088 ; ZR2017QB008) ; 81403051)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000441049900002
出版者	SPRINGER WIEN
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13767
专题	中国科学院西北高原生物研究所
通讯作者	Zhu, Shuyun
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 3.Qufu Normal Univ, Coll Life Sci, Qufu 273165, Shandong, Peoples R China 4.Qilu Univ Technol, Shandong Acad Sci, Shandong Anal & Test Ctr, Shandong Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yan, Xiaolu,Sun, Jing,Zhao, Xian-En,et al. Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission[J]. MICROCHIMICA ACTA,2018,185(9).
APA	Yan, Xiaolu.,Sun, Jing.,Zhao, Xian-En.,Wang, Renjun.,Wang, Xiao.,...&Zhu, Shuyun.(2018).Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission.MICROCHIMICA ACTA,185(9).
MLA	Yan, Xiaolu,et al."Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission".MICROCHIMICA ACTA 185.9(2018).