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Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers
Xia, T; Chen, SL; Chen, SY; Ge, XJ
2005-04-01
发表期刊BIOCHEMICAL GENETICS
卷号43期号:3-4页码:87-101
文章类型Article
摘要Genetic variation of 10 Rhodiola alsia ( Crassulaceae) populations from the Qinghai - Tibet Plateau of China was investigated using intersimple sequence repeat (ISSR) markers. R. alsia is an endemic species of the Qinghai - Tibet Plateau. Of the 100 primers screened, 13 were highly polymorphic. Using these primers, 140 discernible DNA fragments were generated with 112 (80%) being polymorphic, indicating pronounced genetic variation at the species level. Also there were high levels of polymorphism at the population level with the percentage of polymorphic bands (PPB) ranging from 63.4 to 88.6%. Analysis of molecular variance (AMOVA) showed that the genetic variation was mainly found among populations (70.3%) and variance within populations was 29.7%. The main factors responsible for the high level of differentiation among populations are probably the isolation from other populations and clonal propagation of this species. Occasional sexual reproduction might occur in order to maintain high levels of variation within populations. Environmental conditions could also influence population genetic structure as they occur in severe habitats. The strong genetic differentiation among populations in our study indicates that the conservation of genetic variability in R. alsia requires maintenance of as many populations as possible.; Genetic variation of 10 Rhodiola alsia ( Crassulaceae) populations from the Qinghai - Tibet Plateau of China was investigated using intersimple sequence repeat (ISSR) markers. R. alsia is an endemic species of the Qinghai - Tibet Plateau. Of the 100 primers screened, 13 were highly polymorphic. Using these primers, 140 discernible DNA fragments were generated with 112 (80%) being polymorphic, indicating pronounced genetic variation at the species level. Also there were high levels of polymorphism at the population level with the percentage of polymorphic bands (PPB) ranging from 63.4 to 88.6%. Analysis of molecular variance (AMOVA) showed that the genetic variation was mainly found among populations (70.3%) and variance within populations was 29.7%. The main factors responsible for the high level of differentiation among populations are probably the isolation from other populations and clonal propagation of this species. Occasional sexual reproduction might occur in order to maintain high levels of variation within populations. Environmental conditions could also influence population genetic structure as they occur in severe habitats. The strong genetic differentiation among populations in our study indicates that the conservation of genetic variability in R. alsia requires maintenance of as many populations as possible.
关键词Rhodiola Alsia Issrs Genetic Variation Population Structure Qinghai-tibet Plateau
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科领域生物化学
关键词[WOS]SEQUENCE REPEAT POLYMORPHISMS ; RAPD VARIATION ; CONSERVATION GENETICS ; NATURAL-POPULATIONS ; CLONAL DIVERSITY ; ENDANGERED PLANT ; SIZE ; DIFFERENTIATION ; CONSEQUENCES ; DNA
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000227908200001
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1378
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol Evolut & Adaptat, Xining, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, S China Inst Bot, Guangzhou, Peoples R China
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GB/T 7714
Xia, T,Chen, SL,Chen, SY,et al. Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers[J]. BIOCHEMICAL GENETICS,2005,43(3-4):87-101.
APA Xia, T,Chen, SL,Chen, SY,&Ge, XJ.(2005).Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers.BIOCHEMICAL GENETICS,43(3-4),87-101.
MLA Xia, T,et al."Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers".BIOCHEMICAL GENETICS 43.3-4(2005):87-101.
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