A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection

doi:10.1371/journal.pone.0198102

NWIPB OpenIR

	A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection
	Fan, Baolei 1,2; You, Jinmao2 ; Suo, Yourui2 ; Qian, Chunqi 3
	2018-06-06
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	13 期号:6
文章类型	Article
摘要	A new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL(-1), respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.
WOS标题词	Science & Technology
DOI	10.1371/journal.pone.0198102
关键词[WOS]	WATER-SOLUBLE VITAMINS ; MASS-SPECTROMETRY ; ENRICHED DRINK ; MULTIVITAMIN ; TAURINE ; TABLETS ; INGREDIENTS ; RIBOFLAVIN ; METABOLISM ; PYRIDOXINE
收录类别	SCI
语种	英语
项目资助者	Educational Commission of Hubei Province of China(D20142806) ; Plan Projects of Science and Technology of Hubei Province of China(2015CFC777)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000434369400039
出版者	PUBLIC LIBRARY SCIENCE
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13807
专题	中国科学院西北高原生物研究所
通讯作者	Suo, Yourui; Qian, Chunqi
作者单位	1.Hubei Univ Sci & Technol, Xianning, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 3.Michigan State Univ, E Lansing, MI 48824 USA
推荐引用方式 GB/T 7714	Fan, Baolei,You, Jinmao,Suo, Yourui,et al. A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection[J]. PLOS ONE,2018,13(6).
APA	Fan, Baolei,You, Jinmao,Suo, Yourui,&Qian, Chunqi.(2018).A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection.PLOS ONE,13(6).
MLA	Fan, Baolei,et al."A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection".PLOS ONE 13.6(2018).