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A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection
Fan, Baolei1,2; You, Jinmao2; Suo, Yourui2; Qian, Chunqi3
2018-06-06
Source PublicationPLOS ONE
ISSN1932-6203
Volume13Issue:6
SubtypeArticle
AbstractA new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL(-1), respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.
WOS HeadingsScience & Technology
DOI10.1371/journal.pone.0198102
WOS KeywordWATER-SOLUBLE VITAMINS ; MASS-SPECTROMETRY ; ENRICHED DRINK ; MULTIVITAMIN ; TAURINE ; TABLETS ; INGREDIENTS ; RIBOFLAVIN ; METABOLISM ; PYRIDOXINE
Indexed BySCI
Language英语
Funding OrganizationEducational Commission of Hubei Province of China(D20142806) ; Plan Projects of Science and Technology of Hubei Province of China(2015CFC777)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000434369400039
PublisherPUBLIC LIBRARY SCIENCE
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13807
Collection中国科学院西北高原生物研究所
Corresponding AuthorSuo, Yourui; Qian, Chunqi
Affiliation1.Hubei Univ Sci & Technol, Xianning, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China
3.Michigan State Univ, E Lansing, MI 48824 USA
Recommended Citation
GB/T 7714
Fan, Baolei,You, Jinmao,Suo, Yourui,et al. A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection[J]. PLOS ONE,2018,13(6).
APA Fan, Baolei,You, Jinmao,Suo, Yourui,&Qian, Chunqi.(2018).A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection.PLOS ONE,13(6).
MLA Fan, Baolei,et al."A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection".PLOS ONE 13.6(2018).
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