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An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
Huang, Qiong1; Bu, Tong1; Zhang, Wentao1; Yan, Lingzhi1; Zhang, Mengyue1; Yang, Qingfeng1; Huang, Lunjie1; Yang, Baowei1; Hu, Na2; Suo, Yourui2; Wang, Jianlong1; Zhang, Daohong1
2018-10-01
发表期刊FOOD CHEMISTRY
ISSN0308-8146
卷号262页码:48-55
文章类型Article
摘要Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
关键词Immunochromatographic Assay Clenbuterol Bacteria Gold Nanoparticles Signal Amplification
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1016/j.foodchem.2018.04.085
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; LATERAL FLOW IMMUNOASSAY ; BETA-AGONISTS ; SENSITIVE DETECTION ; RAPID DETECTION ; TEST STRIP ; URINE ; RESIDUES ; LIVER
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21675127 ; New Century Excellent Talents in University(NCET-13-0483) ; Fundamental Research Funds for the Northwest A&F University of China(2014YB093 ; 31501560) ; 2452015257)
WOS研究方向Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号WOS:000432652700007
出版者ELSEVIER SCI LTD
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/13819
专题中国科学院西北高原生物研究所
通讯作者Wang, Jianlong; Zhang, Daohong
作者单位1.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810008, Qinghai, Peoples R China
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Huang, Qiong,Bu, Tong,Zhang, Wentao,et al. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier[J]. FOOD CHEMISTRY,2018,262:48-55.
APA Huang, Qiong.,Bu, Tong.,Zhang, Wentao.,Yan, Lingzhi.,Zhang, Mengyue.,...&Zhang, Daohong.(2018).An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.FOOD CHEMISTRY,262,48-55.
MLA Huang, Qiong,et al."An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier".FOOD CHEMISTRY 262(2018):48-55.
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