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An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
Huang, Qiong1; Bu, Tong1; Zhang, Wentao1; Yan, Lingzhi1; Zhang, Mengyue1; Yang, Qingfeng1; Huang, Lunjie1; Yang, Baowei1; Hu, Na2; Suo, Yourui2; Wang, Jianlong1; Zhang, Daohong1
2018-10-01
Source PublicationFOOD CHEMISTRY
ISSN0308-8146
Volume262Pages:48-55
SubtypeArticle
AbstractImmunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
KeywordImmunochromatographic Assay Clenbuterol Bacteria Gold Nanoparticles Signal Amplification
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1016/j.foodchem.2018.04.085
WOS KeywordTANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; LATERAL FLOW IMMUNOASSAY ; BETA-AGONISTS ; SENSITIVE DETECTION ; RAPID DETECTION ; TEST STRIP ; URINE ; RESIDUES ; LIVER
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21675127 ; New Century Excellent Talents in University(NCET-13-0483) ; Fundamental Research Funds for the Northwest A&F University of China(2014YB093 ; 31501560) ; 2452015257)
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000432652700007
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13819
Collection中国科学院西北高原生物研究所
Corresponding AuthorWang, Jianlong; Zhang, Daohong
Affiliation1.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810008, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Huang, Qiong,Bu, Tong,Zhang, Wentao,et al. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier[J]. FOOD CHEMISTRY,2018,262:48-55.
APA Huang, Qiong.,Bu, Tong.,Zhang, Wentao.,Yan, Lingzhi.,Zhang, Mengyue.,...&Zhang, Daohong.(2018).An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.FOOD CHEMISTRY,262,48-55.
MLA Huang, Qiong,et al."An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier".FOOD CHEMISTRY 262(2018):48-55.
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