Knowledge Management System of Northwest Institute of Plateau Biology, CAS
An antioxidant alpha-glucan from Cladina rangiferina (L) Nyl. and its protective effect on alveolar epithelial cells from Pb2+-induced oxidative damage | |
Huang, Xiaojuan1,2,3; Ma, Jianbin4; Wei, Lixin1,2; Song, Jiayin5; Li, Cen1,2; Yang, Hongxia1,2; Du, Yuzhi1,2; Gao, Tingting2; Bi, Hongtao1 | |
2018-06-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
ISSN | 0141-8130 |
卷号 | 112页码:101-109 |
文章类型 | Article |
摘要 | Air pollution is a serious global health problem nowadays. So, it is an emergency to pay sufficient attention to treat and prevent the diseases caused by air pollution, especially respiratory disease and lung damage. Cladina rangiferina (L) Nyl. is an edible lichen that has been used in medicinal diets to treat respiratory and other diseases for over 500 years. In this study, a water-soluble polysaccharide, CRWP-P, was obtained from C rangiferina by hot-water extraction, freeze-thawing separation, and Fehling reagent purification. Structural analysis showed that CRWP-P is a linear alpha-(1 -> 3),(1 -> 4)-D-glucan without branches. Its Mw was determined to be 1.05 x 10(5) Da. Its (1,3)-alpha-D-glucopyranosyl: (1,4)-alpha-D-glucopyranosyl ratio is approximately 1:2. Antioxidant activity assay showed that C. rangiferina polysaccharides, especially CRWP-P. had appreciable DPPH radical-scavenging activity and reducing power. Notably, they could effectively decrease cell breakdown and ROS generation, inhibit lipid peroxidation, increase key antioxidase activity, and promote glutathione redox cycling in Pb2+-oxidative injured A549 alveolar epithelium cells. Overall, the results of this study indicated that C rangiferina polysaccharides, especially CRWP-P, have the potential to be natural antioxidants for the treatment of lung oxidative damage induced by lead of air pollutants. (C) 2018 Elsevier B.V. All rights reserved. |
关键词 | Cladina Rangiferina (l) Nyl. Alpha-glucan Antioxidant Activity Alveolar Epithelium Cell pb2+-oxidative Toxicity Protection |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.ijbiomac.2018.01.154 |
关键词[WOS] | IN-VITRO ANTIOXIDANT ; STRUCTURAL-CHARACTERIZATION ; LIPID-PEROXIDATION ; PARTICULATE MATTER ; CETRARIA-ISLANDICA ; POLYSACCHARIDES ; LICHENS ; DERIVATIVES ; ASSAY ; LEAD |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(31400310 ; Applying Basic Research Program of Qinghai Province(2015-ZJ-727) ; Key Laboratory Special Development Program of Qinghai Province(2017-ZJ-Y08) ; Natural Science Foundation of Tianjin City(15JCQJC07500) ; 51408405) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
WOS记录号 | WOS:000430522400012 |
出版者 | ELSEVIER SCIENCE BV |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/13833 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Gao, Tingting; Bi, Hongtao |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xining Rd, Xining 810008, Qinghai, Peoples R China 3.Sichuan Univ, Natl Key Lab Biotherapy, Chengdu, Sichuan, Peoples R China 4.Qinghai Normal Univ, Sch Life & Geog Sci, Xining, Qinghai, Peoples R China 5.Tianjin Univ, Tianjin Key Lab Architectural Phys & Environm Tec, Tianjin, Peoples R China |
推荐引用方式 GB/T 7714 | Huang, Xiaojuan,Ma, Jianbin,Wei, Lixin,et al. An antioxidant alpha-glucan from Cladina rangiferina (L) Nyl. and its protective effect on alveolar epithelial cells from Pb2+-induced oxidative damage[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,112:101-109. |
APA | Huang, Xiaojuan.,Ma, Jianbin.,Wei, Lixin.,Song, Jiayin.,Li, Cen.,...&Bi, Hongtao.(2018).An antioxidant alpha-glucan from Cladina rangiferina (L) Nyl. and its protective effect on alveolar epithelial cells from Pb2+-induced oxidative damage.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,112,101-109. |
MLA | Huang, Xiaojuan,et al."An antioxidant alpha-glucan from Cladina rangiferina (L) Nyl. and its protective effect on alveolar epithelial cells from Pb2+-induced oxidative damage".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 112(2018):101-109. |
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