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Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis
Suo, YR; Wang, HL; Li, YL; You, JM; Wang, HQ
2004-11-01
发表期刊CHROMATOGRAPHIA
卷号60期号:9-10页码:589-595
文章类型Article
摘要A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. The separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mM boric acid, 30 mM SDS and 2.5% acetonitrile, adjusted to pH 10.7 with NaOH. Applied potential was 21 kV. The temperature of the capillary was maintained at 25 degreesC by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. The established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of Rhodiola with satisfactory results.; A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. The separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mM boric acid, 30 mM SDS and 2.5% acetonitrile, adjusted to pH 10.7 with NaOH. Applied potential was 21 kV. The temperature of the capillary was maintained at 25 degreesC by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. The established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of Rhodiola with satisfactory results.
关键词Capillary Electrophoresis Pharmacologically Active Compounds Rhodiola Crenucata Rhodiola Quadrifida
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域生物化学
关键词[WOS]HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; SEPARATION ; PEPTIDES ; PHASE ; ELECTROCHROMATOGRAPHY
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000225079600017
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1386
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810008, Peoples R China
2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Suo, YR,Wang, HL,Li, YL,et al. Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis[J]. CHROMATOGRAPHIA,2004,60(9-10):589-595.
APA Suo, YR,Wang, HL,Li, YL,You, JM,&Wang, HQ.(2004).Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis.CHROMATOGRAPHIA,60(9-10),589-595.
MLA Suo, YR,et al."Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis".CHROMATOGRAPHIA 60.9-10(2004):589-595.
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