Molecular characterization of LMW glutenin genes from Taeniatherum Nevski

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	Molecular characterization of LMW glutenin genes from Taeniatherum Nevski
	Han, Chang 1; Yan, Ze-Hong 1,2; Dai, Shou-Fen 1,2; Liu, Deng-Cai 1,2,3; Wei, Yu-Ming 1,2; Zheng, You-Liang 1,2; Lan, Xiu-Jin 1,2; Peng, Yuan-Ying 1,2
	2011-10-01
发表期刊	GENETIC RESOURCES AND CROP EVOLUTION
ISSN	0925-9864
卷号	58 期号:7 页码:1029-1039
文章类型	Article
摘要	We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.; We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.
关键词	Gene Sequencing Lmw Glutenin Sequence Analysis Taeniatherum
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SUBUNIT GENES ; AEGILOPS-TAUSCHII ; HEXAPLOID WHEAT ; DURUM-WHEAT ; GLU-3 LOCI ; SEQUENCE ; CLONING ; BREAD ; IDENTIFICATION ; ORGANIZATION
收录类别	SCI
语种	英语
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agronomy ; Plant Sciences
WOS记录号	WOS:000294956900008
引用统计
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1506
专题	中国科学院西北高原生物研究所
作者单位	1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Yaan 625014, Sichuan, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Han, Chang,Yan, Ze-Hong,Dai, Shou-Fen,et al. Molecular characterization of LMW glutenin genes from Taeniatherum Nevski[J]. GENETIC RESOURCES AND CROP EVOLUTION,2011,58(7):1029-1039.
APA	Han, Chang.,Yan, Ze-Hong.,Dai, Shou-Fen.,Liu, Deng-Cai.,Wei, Yu-Ming.,...&Peng, Yuan-Ying.(2011).Molecular characterization of LMW glutenin genes from Taeniatherum Nevski.GENETIC RESOURCES AND CROP EVOLUTION,58(7),1029-1039.
MLA	Han, Chang,et al."Molecular characterization of LMW glutenin genes from Taeniatherum Nevski".GENETIC RESOURCES AND CROP EVOLUTION 58.7(2011):1029-1039.

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