Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii | |
Xue, Chun-Ying ; Li, De-Zhu ; Lu, Jin-Mei ; Yang, Jun-Bo ; Liu, Jian-Quan | |
2006-10-01 | |
发表期刊 | PLANTA MEDICA
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摘要 | Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.; Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/15143 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,et al. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii[J]. PLANTA MEDICA, Xue ChunYing, Li DeZhu,Lu JinMei, Yang JunBo, Liu JianQuan. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. PLANTA MEDICA,2006,72(13):1223-1226,2006. |
APA | Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,Yang, Jun-Bo,&Liu, Jian-Quan.(2006).Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii.PLANTA MEDICA. |
MLA | Xue, Chun-Ying,et al."Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii".PLANTA MEDICA (2006). |
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